Anti-LRP5 + LRP6 antibody [1A12]
| Name | Anti-LRP5 + LRP6 antibody [1A12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab2572 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 1A12 |
| Applications | FA WB ICC/IF ICC/IF FC |
| Species Reactivities | Rat, Human, Mouse |
| Antigen | Synthetic peptide: DTGTDRIEVTR Synthetic peptide: DTGTDRIEVTR (Human) |
| Blocking Peptide | LRP5 + LRP6 peptide |
| Description | Mouse Monoclonal |
| Gene | LRP6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab2572 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2572, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HepG2 cells stained with ab2572 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2572, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% MeOH/permeabilized in 0.1% PBS-Tween used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/17292_LRP5-LRP6-Primary-antibodies-ab2572-3.jpg)
Overlay histogram showing HepG2 cells stained with ab2572 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2572, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% MeOH/permeabilized in 0.1% PBS-Tween used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Activation of the Wnt pathway plays a pathogenic role in diabetic retinopathy in - Activation of the Wnt pathway plays a pathogenic role in diabetic retinopathy in
Chen Y, Hu Y, Zhou T, Zhou KK, Mott R, Wu M, Boulton M, Lyons TJ, Gao G, Ma JX. Am J Pathol. 2009 Dec;175(6):2676-85.
Bone density ligand, Sclerostin, directly interacts with LRP5 but not LRP5G171V - Bone density ligand, Sclerostin, directly interacts with LRP5 but not LRP5G171V
Ellies DL, Viviano B, McCarthy J, Rey JP, Itasaki N, Saunders S, Krumlauf R. J Bone Miner Res. 2006 Nov;21(11):1738-49.
WNT and beta-catenin signalling: diseases and therapies. - WNT and beta-catenin signalling: diseases and therapies.
Moon RT, Kohn AD, De Ferrari GV, Kaykas A. Nat Rev Genet. 2004 Sep;5(9):691-701.