LRP6

Gene Symbol LRP6
Entrez Gene 4040
Alt Symbol ADCAD2
Species Human
Gene Type protein-coding
Description low density lipoprotein receptor-related protein 6
Other Description LRP-6|low-density lipoprotein receptor-related protein 6
Swissprots O75581 Q17RZ2
Accessions AAD21410 CCF76957 CDM49020 EAW96253 O75581 AA126261 AB209683 BAD92920 AF074264 AAC33006 AK074543 BAG51971 AK074902 BAG52027 AK094666 AK124760 BC029941 BC117136 AAI17137 BC126405 AAI26406 BC143725 AAI43726 BP229563 XM_006719078 XP_006719141 XM_011520671 XP_011518973 XM_011548873 XP_011547175 XM_011548874 XP_011547176 XR_429034 XR_429035 XR_953254 XR_953255 NM_002336 NP_002327
Function Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity). {ECO:0000250}.
Subcellular Location Membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Note=On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD (By similarity). {ECO:0000250}.
Tissue Specificity Widely coexpressed with LRP5 during embryogenesis and in adult tissues.
Top Pathways Wnt signaling pathway

Phospho-LRP6 (Ser1490) Antibody - 2568 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of total cell lysates from HeLa cells untreated, treated 5 hours with conditioned medium (CM) from L cells, or treated 5 hours with conditioned medium from L cells overexpressing Wnt3a, using Phospho-LRP6 (Ser1490) Antibody #2568.

LRP6 (C5C7) Rabbit mAb - 2560 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C5C7
Western blot analysis of total cell lysates from HepG2, HeLa and Rat2 cells using LRP6 (C5C7) Rabbit mAb.

LRP6 (C47E12) Rabbit mAb - 3395 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C47E12
Western blot analysis of extracts from Hep G2 and HeLa cells using LRP6 (C47E12) Rabbit mAb.

LRP5/6 (1A12) - sc-57354 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

LRP6 (C-10) - sc-25317 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LRP6 (C-10): sc-25317. Western blot analysis of LRP6 expression in Jurkat whole cell lysate.

LRP6 (C-20) - sc-17984 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

LRP6 (H-105) - sc-134462 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

LRP6 (E-20) - sc-17982 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

LRP6 (H-300) - sc-15399 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LRP6 (H-300): sc-15399. Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic and membrane staining of glandular cells.

Anti-LRP5 + LRP6 antibody - ab51910 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LRP5 + LRP6 antibody (ab51910) at 4 µg/mlLane 1 : Jurkat cell lysate at 100 µgLane 2 : Jurkat cell lysate at 50 µgSecondaryHRP conjugated goat anti rabbit IgG

Anti-LRP5 + LRP6 antibody [1A12] - ab2572 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1A12
ICC/IF image of ab2572 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2572, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-LRP5 + LRP6 antibody [1A12] - ab52215 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1A12
Anti-LRP5 + LRP6 antibody [1A12] (ab52215) at 2 µg/ml + A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-LRP6 (phospho S1490) antibody [EP2360Y] - ab76417 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2360Y
All lanes : Anti-LRP6 (phospho S1490) antibody [EP2360Y] (ab76417) at 1/1000 dilutionLane 1 : HeLa cell lysates, untreatedLane 2 : HeLa cell lysates treated with CalyculinLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-LRP6 antibody - ab66156 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LRP6 antibody (ab66156) at 1/500 dilutionLane 1 : 3T3 cell lysateLane 2 : 3T3 cell lysate preincubated with immunising peptide.Lysates/proteins at 5 µg per lane.

Anti-LRP6 antibody - ab24386 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Ab24386 (5ug/ml) staining human LRP6 in human liver tissue by immunohistochemistry using paraffin embedded tissue. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. In paraffin embedded Human Liver shows strong cytoplasmic staining in hepatocytes and cytoplasmic staining in bile ductulus cells.

Anti-LRP6 antibody - ab118490 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab118490 at 10&microg/ml staining LRP6 in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-LRP6 antibody - ab192655 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-LRP6 antibody - ab93118 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-LRP6 antibody [1C10] - ab75358 from Abcam

Prices $382.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C10

Anti-LRP6 antibody [EPR2423(2)] - ab134146 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2423(2)
All lanes : Anti-LRP6 antibody [EPR2423(2)] (ab134146) at 1/1000 dilutionLane 1 : HeLa cell lysates Lane 2 : HepG2 cell lysates Lane 3 : 293T cell lysates Lane 4 : Jurkat cell lysates Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-LRP6 antibody - N-terminal - ab187130 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-LRP6 antibody - N-terminal (ab187130) at 1/1000 dilution + Mouse thymus whole cell extracts

Anti-LRP6 Antibody (aa1546-1560) - LS-C121247 from LifeSpan Bioscience

Prices $680.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-LRP6 Antibody (C-Terminus) - LS-C123986 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-LRP6 Antibody - LS-C130478 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal

Anti-LRP6 Antibody - LS-C191466 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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