Anti-LRP5 antibody
| Name | Anti-LRP5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab36121 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB IP |
| Species Reactivities | Rabbit, Human, Mouse, Rat |
| Antigen | Synthetic peptide: ERVEKTTGDKRT , corresponding to Internal sequence amino acids 1178-1189 of Human LRP5 Run BLAST with Run BLAST with |
| Description | Goat Polyclonal |
| Gene | LRP5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab36121 at 1ug/ml staining LRP5 in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9). HRP-staining procedure was used for detection.
ICC/IF image of ab36121 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36121, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab36121 staining LRP5 in Human kidney tissue at a concentration of 4 µg/ml by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6.0 was performed, and the antibody was detected with HRP-staining.
Product References
A novel signaling pathway regulates colon cancer angiogenesis through Norrin. - A novel signaling pathway regulates colon cancer angiogenesis through Norrin.
Planutis K, Planutiene M, Holcombe RF. Sci Rep. 2014 Jul 9;4:5630.
Fas-associated factor 1 antagonizes Wnt signaling by promoting beta-catenin - Fas-associated factor 1 antagonizes Wnt signaling by promoting beta-catenin
Zhang L, Zhou F, van Laar T, Zhang J, van Dam H, Ten Dijke P. Mol Biol Cell. 2011 May;22(9):1617-24.