Anti-LRP5 antibody
| Name | Anti-LRP5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38311 |
| Prices | $385.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to Human LRP5 aa 1538-1567 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | LRP5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-LRP5 antibody (ab38311) at 1/1000 dilutionLane 1 : recombinant human LRP5 transfected 293 cell lysatesLane 2 : mouse LRP5 transfected 293 cell lysatesLysates/proteins at 35 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling LRP5 with ab38311. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
ICC/IF image of ab38311 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38311, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
PPARbeta/delta governs Wnt signaling and bone turnover. - PPARbeta/delta governs Wnt signaling and bone turnover.
Scholtysek C, Katzenbeisser J, Fu H, Uderhardt S, Ipseiz N, Stoll C, Zaiss MM, Stock M, Donhauser L, Bohm C, Kleyer A, Hess A, Engelke K, David JP, Djouad F, Tuckermann JP, Desvergne B, Schett G, Kronke G. Nat Med. 2013 May;19(5):608-13.
Mouse Prkar1a haploinsufficiency leads to an increase in tumors in the Trp53+/- - Mouse Prkar1a haploinsufficiency leads to an increase in tumors in the Trp53+/-
Almeida MQ, Muchow M, Boikos S, Bauer AJ, Griffin KJ, Tsang KM, Cheadle C, Watkins T, Wen F, Starost MF, Bossis I, Nesterova M, Stratakis CA. Hum Mol Genet. 2010 Apr 15;19(8):1387-98.