All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/mlLane 1 : GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agaroseLane 2 : GFP-LRRK2 mutant D2017A transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agaroseLane 3 : GFP-LRRK2 mutant S1292A/G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agaroseLane 4 : GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agaroseLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit (IRDye 800) at 1/10000 dilution
All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/mlLane 1 : LRRK2 mutant G2019S/S1292A transfected HEK293 lysateLane 2 : LRRK2 mutant G2019S transfected HEK293 inhibitor treated lysateLane 3 : LRRK2 mutant G2019S transfected HEK293 lysateLane 4 : LRRK2 mutant S1292A transfected HEK293 lysateLane 5 : LRRK2 wt transfected HEK293 inhibitor treated lysateLane 6 : LRRK2 wt transfected HEK293 lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2500 dilution