LRRK2

Gene Symbol LRRK2
Entrez Gene 120892
Alt Symbol AURA17, DARDARIN, PARK8, RIPK7, ROCO2
Species Human
Gene Type protein-coding
Description leucine-rich repeat kinase 2
Other Description augmented in rheumatoid arthritis 17|leucine-rich repeat serine/threonine-protein kinase 2
Swissprots Q6ZS50 Q5S007 Q8NCX9 A6NJU2
Accessions Q5S007 AK026776 AK122640 AK127729 AK131537 AL832453 AL834529 CAD39185 AY792511 AAV63975 BC117180 AAI17181 BM285390 BR000408 FAA00322 BU634341 BX640975 CAE45989 CN294292 XM_005268629 XP_005268686 XM_011537877 XP_011536179 XM_011537878 XP_011536180 XM_011537879 XP_011536181 XM_011537880 XP_011536182 XM_011537881 XP_011536183 XM_011537882 XP_011536184 NM_198578 NP_940980
Function Positively regulates autophagy through a calcium- dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease. {ECO:0000269|PubMed:17114044, ECO:0000269|PubMed:18230735, ECO:0000269|PubMed:20949042, ECO:0000269|PubMed:21850687, ECO:0000269|PubMed:22012985, ECO:0000269|PubMed:23395371, ECO:0000269|PubMed:24687852}.
Subcellular Location Membrane; Peripheral membrane protein. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum {ECO:0000250}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000269|PubMed:24687852}; Peripheral membrane protein {ECO:0000269|PubMed:24687852}; Cytoplasmic side {ECO:0000269|PubMed:24687852}. Endosome {ECO:0000250}. Lysosome {ECO:0000250}. Mitochondrion outer membrane {ECO:0000250}. Mitochondrion inner membrane {ECO:0000250}. Mitochondrion matrix {ECO:0000250}. Note=Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal p
Tissue Specificity Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. {ECO:0000269|PubMed:15541308, ECO:0000269|PubMed:15541309, ECO:0000269|PubMed:16532471, ECO:0000269|PubMed:17120249}.

LRRK2 (D18E12) Rabbit mAb - 13046 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D18E12
Western blot analysis of extracts from U-87 MG and A172 cells, and mouse brain using LRRK2 (D18E12) Rabbit mAb.

LRRK2 Antibody - 5559 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from mouse LRRK2 -/- and wild-type tissue using LRRK2 Antibody (upper) and TrkB (80E3) Rabbit mAb #4603 (lower). (Mouse wild-type and LRRK2 -/- brains were kindly provided by Dr. Jie Shen, Brigham and Women's Hospital and Harvard Medical School, Boston, MA).

LRRK2 Antibody - 2567 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HEK-293 cells, transfected with GFP alone (lane 1) or GFP-LRRK2 (lane 2), using LRRK2 Antibody.

LRRK2 (C-13) - sc-48733 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LRRK2 (C-13): sc-48733. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

LRRK2 (M-300) - sc-66954 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LRRK2 (M-300): sc-66954. Western blot analysis of LRRK2 expression in EOC 20 whole cell lysate.

LRRK2 (133AT1218) - sc-130159 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - ab203181 from Abcam

Prices $204.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MJFR-19-7-8
All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/mlLane 1 : GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agaroseLane 2 : GFP-LRRK2 mutant D2017A transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agaroseLane 3 : GFP-LRRK2 mutant S1292A/G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agaroseLane 4 : GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agaroseLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit (IRDye 800) at 1/10000 dilution

Anti-LRRK2 (phospho S910) antibody [UDD1 15(3)] - ab133449 from Abcam

Prices $67.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone UDD1 15(3)
All lanes : Anti-LRRK2 (phospho S910) antibody [UDD1 15(3)] (ab133449) at 1/1000 dilutionLane 1 : GFP tagged WT LRRK2 lysate at 5 µgLane 2 : GFP LRRK2 S910A lysate at 5 µgLane 3 : GFP LRRK2 S935A lysate at 5 µgLane 4 : Lymphoblastoid lysate at 30 µgLane 5 : Lymphoblastoid lysate at 30 µgLane 6 : Lymphoblastoid lysate from LRRK2 IN1 treated cells at 30 µgLane 7 : Lymphoblastoid lysate from LRRK2 IN1 treated cells at 30 µgSecondaryHRP conjugated goat anti-rabbit antibody at 1/2000 dilution

Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - ab133450 from Abcam

Prices $67.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone UDD2 10(12)
All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/1000 dilution (unpurified)Lane 1 : GFP LRRK2 lysate at 5 µgLane 2 : GFP LRRK2 S910A lysate at 5 µgLane 3 : GFP LRRK2 S935A lysate at 5 µgLane 4 : LRRK2 WT MEF lysate at 20 µgLane 5 : LRRK2 WT MEF lysate from LRRK2 IN1 treated cells at 20 µgLane 6 : LRRK2 KO MEF lysate at 20 µgLane 7 : LRRK2 KO MEF lysate from LRRK2 IN1 treated cells at 20 µgLane 8 : Lymphoblastoid lysate at 30 µgLane 9 : Lymphoblastoid lysate from LRRK2 IN1 treated cells at 30 µgSecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)] - ab169521 from Abcam

Prices $204.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MJF-R11 (75-1)
Lanes 1 - 2 :  Anti-DDDDK tag antibodyLanes 3 - 4 :  Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)] (ab169521) at 1/2000 dilutionLanes 5 - 6 :  Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)] (ab169521) at 1/10000 dilutionLane 1 : HEK293T cells stably expressed with wild-type DDDDK-LRRK2Lane 2 : HEK293T cells stably expressed with mutant DDDDK-LRRK2Lane 3 : HEK293T cells stably expressed with wild-type DDDDK-LRRK2Lane 4 : HEK293T cells stably expressed with mutant DDDDK-LRRK2Lane 5 : HEK293T cells stably expressed with wild-type DDDDK-LRRK2Lane 6 : HEK293T cells stably expressed with mutant DDDDK-LRRK2

Anti-LRRK2 (phospho S973) antibody [MJF-R12 (37-1)] - ab181364 from Abcam

Prices $204.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MJF-R12 (37-1)
All lanes : Anti-LRRK2 (phospho S973) antibody [MJF-R12 (37-1)] (ab181364) at 1/1000 dilutionLane 1 : Lysate from HEK293 cells expressing LRRK2Lane 2 : Lysate from HEK293 cells expressing LRRK2(S973A)

Anti-LRRK2 (phospho T1410) antibody [MJFR4-25-5] - ab140107 from Abcam

Prices $204.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MJFR4-25-5
All lanes : Anti-LRRK2 (phospho T1410) antibody [MJFR4-25-5] (ab140107) at 1/1000 dilutionLane 1 : LRRK2 wild type transfected 293T in vitro auto-phosphorylation Lane 2 : LRRK2 T1410A in vitro auto-phosphorylation

Anti-LRRK2 (phospho T1491) antibody [MJFR5-88-3] - ab140106 from Abcam

Prices $204.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MJFR5-88-3
All lanes : Anti-LRRK2 (phospho T1491) antibody [MJFR5-88-3] (ab140106) at 1/1000 dilutionLane 1 : Wildtype of LRRK2 + ATPLane 2 : Wildtype of LRRK2 - ATPLane 3 : Mutant construct of T1491A (A replace T) + ATPLane 4 : Mutant construct of T1491A (A replace T) - ATPLane 5 : Mutant construct of G2019S (S replace G) + ATPLane 6 : Mutant construct of G2019S (S replace G) - ATPLane 7 : Kinase dead + ATPLane 8 : Kinase dead - ATP

Anti-LRRK2 (phospho T1503) antibody [ MJF-R6 (227-1a)] - ab154423 from Abcam

Prices $204.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MJF-R6 (227-1a)
Lanes 1 - 2 :  Anti-LRRK2 (phospho T1503) antibody [ MJF-R6 (227-1a)] (ab154423) at 1/1000 dilutionLanes 3 - 4 :  Anti-LRRK2 (phospho T1503) antibody [ MJF-R6 (227-1a)] (ab154423) at 1/5000 dilutionLanes 5 - 6 :  Monoclonal antibody against total LRRK2Lane 1 : HEK293FT cells, transiently transfected with wild-type Human LRRK2 immunoprecipitated for LRRK2 and subjected to an in vitro kinase assayLane 2 : HEK293FT cells, transiently transfected with HEK293FT cells, transiently transfected with DDDDK-tagged Human LRRK2(T1503A) immunoprecipitated for LRRK2(T1503A) and subjected to an in vitro kinase assay.Lane 3 : HEK293FT cells, transiently transfected with wild-type Human LRRK2 immunoprecipitated for LRRK2 and subjected to an in vitro kinase assayLane 4 : HEK293FT cells, transiently transfected with HEK293FT cells, transiently transfected with DDDDK-tagged Human LRRK2(T1503A) immunoprecipitated for LRRK2(T1503A) and subjected to an in vitro kinase assay.Lane 5 : HEK293FT cells, transient

Anti-LRRK2 (phospho T2483) antibody [MJF-R8 (21-2e)] - ab156577 from Abcam

Prices $204.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MJF-R8 (21-2e)
All lanes : Anti-LRRK2 (phospho T2483) antibody [MJF-R8 (21-2e)] (ab156577) at 1/1000 dilutionLane 1 : HEK293 cells transiently transfected with wild type LRRK2Lane 2 : HEK293 cells transiently transfected with T2483Lane 3 : HEK293 cells transiently transfected with G2019SLane 4 : HEK293 cells transiently transfected with Kinase deadLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-LRRK2 antibody - ab115590 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab115590, at 5 µg/ml, staining LRRK2 in formalin fixed, paraffin embedded Human brain, cortex by Immunohistochemistry.

Anti-LRRK2 antibody - ab189644 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling LRRK2 with ab189644 at 10 µg/ml. Detection utilised biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-LRRK2 antibody - ab60155 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab60155, at a 1/10-1/50 dilution, staining LRRK2 in formalin fixed, paraffin embedded human hepatocarcinoma tissue from smooth muscle (peroxidase-conjugated secondary antibody; DAB staining).

Anti-LRRK2 antibody - ab31546 from Abcam

Prices $370.00
Sizes 100 µl
Host Sheep
Clonality Polyclonal

Anti-LRRK2 antibody - ab57329 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57329 LRRK2 antibody at 1ug/ml.

Anti-LRRK2 antibody (Biotin) - ab82195 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-LRRK2 antibody [MC.028.83.76.242] - ab130277 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone MC.028.83.76.242
ab130277, at 1/50 dilution, staining LRRK2 in formalin-fixed, paraffin-embedded Human pancreas tissue by Immunohistochemistry.

Anti-LRRK2 antibody [MJFF2 (c41-2)] - ab133474 from Abcam

Prices $204.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MJFF2 (c41-2)
Immunohistochemical analysis of mouse brain tissue, staining LRRK2 with ab133474.Antigen retrieval was performed by heat mediation and tissue was blocked with 2% goat serum. Sections were incubated with primary antibody (1/200) overnight at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Anti-LRRK2 antibody [MJFF3 (c69-6)] - ab133475 from Abcam

Prices $204.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MJFF3 (c69-6)
All lanes : Anti-LRRK2 antibody [MJFF3 (c69-6)] (ab133475) at 1/1000 dilutionLane 1 : HEK293 cell lysate transfected with 3*Flag vectorLane 2 : HEK293 cell lysate transfected with 3*Flag wild type, full length LRRK2Lysates/proteins at 10 µg per lane.SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution

Anti-LRRK2 antibody [MJFF4 (c81-8)] - ab133476 from Abcam

Prices $204.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MJFF4 (c81-8)
All lanes : Anti-LRRK2 antibody [MJFF4 (c81-8)] (ab133476) at 1/1000 dilutionLane 1 : HEK293 cell lysate transfected with 3*Flag vectorLane 2 : HEK293 cell lysate transfected with 3*Flag wild type Human LRRK2 full lengthLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
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