Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)]

• Supplier: Abcam
• Catalog: ab133450
• Prices: $67.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: UDD2 10(12)
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human LRRK2 (phospho S935)
• Description: Rabbit Monoclonal
• Gene: LRRK2
• Conjugate: Unconjugated

Product images

All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/1000 dilution (unpurified)Lane 1 : GFP LRRK2 lysate at 5 µgLane 2 : GFP LRRK2 S910A lysate at 5 µgLane 3 : GFP LRRK2 S935A lysate at 5 µgLane 4 : LRRK2 WT MEF lysate at 20 µgLane 5 : LRRK2 WT MEF lysate from LRRK2 IN1 treated cells at 20 µgLane 6 : LRRK2 KO MEF lysate at 20 µgLane 7 : LRRK2 KO MEF lysate from LRRK2 IN1 treated cells at 20 µgLane 8 : Lymphoblastoid lysate at 30 µgLane 9 : Lymphoblastoid lysate from LRRK2 IN1 treated cells at 30 µgSecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/5000 dilution (purified)Lane 1 : WT-LRRK2 cell lysate - untreatedLane 2 : WT-LRRK2 cell lysate - treated with Lambda phosphataseLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/1000 dilution (purified) + Raw264.7 cell lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling LRRK2 (phospho S935) with purified ab133450 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/100) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

Product References

Membrane recruitment of endogenous LRRK2 precedes its potent regulation of - Membrane recruitment of endogenous LRRK2 precedes its potent regulation of

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Lack of correlation between the kinase activity of LRRK2 harboring - Lack of correlation between the kinase activity of LRRK2 harboring

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A Parkinson's disease gene regulatory network identifies the signaling protein - A Parkinson's disease gene regulatory network identifies the signaling protein

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Deficient production of reactive oxygen species leads to severe chronic - Deficient production of reactive oxygen species leads to severe chronic

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LRRK2 kinase activity regulates synaptic vesicle trafficking and neurotransmitter - LRRK2 kinase activity regulates synaptic vesicle trafficking and neurotransmitter

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In silico, in vitro and cellular analysis with a kinome-wide inhibitor panel - In silico, in vitro and cellular analysis with a kinome-wide inhibitor panel

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Genetic and pharmacological evidence that G2019S LRRK2 confers a hyperkinetic - Genetic and pharmacological evidence that G2019S LRRK2 confers a hyperkinetic

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Arsenite stress down-regulates phosphorylation and 14-3-3 binding of leucine-rich - Arsenite stress down-regulates phosphorylation and 14-3-3 binding of leucine-rich

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Kinase inhibitors arrest neurodegeneration in cell and C. elegans models of LRRK2 - Kinase inhibitors arrest neurodegeneration in cell and C. elegans models of LRRK2

Yao C, Johnson WM, Gao Y, Wang W, Zhang J, Deak M, Alessi DR, Zhu X, Mieyal JJ, Roder H, Wilson-Delfosse AL, Chen SG. Hum Mol Genet. 2013 Jan 15;22(2):328-44.

Inhibition of LRRK2 kinase activity stimulates macroautophagy. - Inhibition of LRRK2 kinase activity stimulates macroautophagy.

Manzoni C, Mamais A, Dihanich S, Abeti R, Soutar MP, Plun-Favreau H, Giunti P, Tooze SA, Bandopadhyay R, Lewis PA. Biochim Biophys Acta. 2013 Dec;1833(12):2900-10. doi: