Lanes 1 - 2 : Anti-DDDDK tag antibodyLanes 3 - 4 : Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)] (ab169521) at 1/2000 dilutionLanes 5 - 6 : Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)] (ab169521) at 1/10000 dilutionLane 1 : HEK293T cells stably expressed with wild-type DDDDK-LRRK2Lane 2 : HEK293T cells stably expressed with mutant DDDDK-LRRK2Lane 3 : HEK293T cells stably expressed with wild-type DDDDK-LRRK2Lane 4 : HEK293T cells stably expressed with mutant DDDDK-LRRK2Lane 5 : HEK293T cells stably expressed with wild-type DDDDK-LRRK2Lane 6 : HEK293T cells stably expressed with mutant DDDDK-LRRK2