Lanes 1 - 2 : Anti-LRRK2 (phospho T1503) antibody [ MJF-R6 (227-1a)] (ab154423) at 1/1000 dilutionLanes 3 - 4 : Anti-LRRK2 (phospho T1503) antibody [ MJF-R6 (227-1a)] (ab154423) at 1/5000 dilutionLanes 5 - 6 : Monoclonal antibody against total LRRK2Lane 1 : HEK293FT cells, transiently transfected with wild-type Human LRRK2 immunoprecipitated for LRRK2 and subjected to an in vitro kinase assayLane 2 : HEK293FT cells, transiently transfected with HEK293FT cells, transiently transfected with DDDDK-tagged Human LRRK2(T1503A) immunoprecipitated for LRRK2(T1503A) and subjected to an in vitro kinase assay.Lane 3 : HEK293FT cells, transiently transfected with wild-type Human LRRK2 immunoprecipitated for LRRK2 and subjected to an in vitro kinase assayLane 4 : HEK293FT cells, transiently transfected with HEK293FT cells, transiently transfected with DDDDK-tagged Human LRRK2(T1503A) immunoprecipitated for LRRK2(T1503A) and subjected to an in vitro kinase assay.Lane 5 : HEK293FT cells, transient