Total extracts from mouse thymus were immunoprecipitated (IP) with IgG(Lanes 1 and 3) or ab134997 (Lanes 3 and 4) using conventional IP conditions or cross-linked chromatin immunoprecipitation (ChIP) conditions; followed by WB with ab134997 at RT for 2 h.
Lanes 1 - 2 : Anti-LSD2 / AOF1 antibody - ChIP Grade (ab134997)Lane 3 : Anti-ACTBL2 antibody (ab134977)Lane 1 : Mouse Thymus LysateLane 2 : Human HepG2 cell lysateLane 3 : Human HeLa cell Lysate
Anti-LSD2 / AOF1 antibody - ChIP Grade (ab134997) at 1/1000 dilution + Total cell extracts from Human HeLa