Anti-LXR beta antibody
| Name | Anti-LXR beta antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab76983 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | ELISA WB ICC/IF ICC/IF FC |
| Species Reactivities | Rat, Human |
| Antigen | Recombinant full length human LXR beta protein (AAH07790) with a 26 kDa tag |
| Description | Mouse Monoclonal |
| Gene | NR1H2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-LXR beta antibody (ab76983) at 5 µg/ml + IMR-32 cell lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Immunofluorescence analysis of LXR beta expression in HeLa cells using 10 µg/ml of ab76983
Detection limit for ab76983 in Sandwich ELISA (using recombinant protein) is approximately 0.03 ng/ml as a capture antibody.
![Overlay histogram showing HeLa cells stained with ab76983 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76983, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/17913_LXR-beta-Primary-antibodies-ab76983-1.jpg)
Overlay histogram showing HeLa cells stained with ab76983 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76983, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
Normalizing glycosphingolipids restores function in CD4+ T cells from lupus - Normalizing glycosphingolipids restores function in CD4+ T cells from lupus
McDonald G, Deepak S, Miguel L, Hall CJ, Isenberg DA, Magee AI, Butters T, Jury EC. J Clin Invest. 2014 Feb;124(2):712-24.
Alterations of LXRalpha and LXRbeta expression in the hypothalamus of - Alterations of LXRalpha and LXRbeta expression in the hypothalamus of
Kruse MS, Rey M, Vega MC, Coirini H. J Endocrinol. 2012 Oct;215(1):51-8.