ICC/IF image of ab129080 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129080 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
![All lanes : Anti-Lysyl tRNA synthetase antibody [EPR7921] (ab129080) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : HepG2 cell lysateLane 5 : Caco 2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/18223_Lysyl-tRNA-synthetase-Primary-antibodies-ab129080-1.jpg)
All lanes : Anti-Lysyl tRNA synthetase antibody [EPR7921] (ab129080) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : HepG2 cell lysateLane 5 : Caco 2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
ab129080, at a dilution of 1/50, staining Lysyl tRNA synthetase in paraffin embedded Human colon tissue by Immunohistochemistry.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD