KARS

Gene Symbol KARS
Entrez Gene 3735
Alt Symbol CMTRIB, DFNB89, KARS1, KARS2, KRS
Species Human
Gene Type protein-coding
Description lysyl-tRNA synthetase
Other Description lysRS|lysine tRNA ligase|lysine--tRNA ligase
Swissprots D3DUK4 Q96J25 O14946 Q9HB23 Q15046 A8MSK1
Accessions EAW95619 EAW95620 EAW95621 EAW95622 EAW95623 EAW95624 Q15046 AA503390 AF285758 AAG30114 AK126584 BAC86604 AK309168 AK315687 BC004132 AAH04132 BC013246 BM781594 D31890 BAA06688 D32053 BAA22084 DQ892301 ABM83227 DQ895500 ABM86426 NM_001130089 NP_001123561 NM_005548 NP_005539
Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. {ECO:0000269|PubMed:15851690, ECO:0000269|PubMed:5338216}.
Subcellular Location Isoform Cytoplasmic: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted. Note=Secretion is induced by TNF-alpha.
Top Pathways Aminoacyl-tRNA biosynthesis

LysRS (D16D1) Rabbit mAb - 9990 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D16D1
Western blot analysis of extracts from various cell lines using LysRS (D16D1) Rabbit mAb.

LysRS Antibody - 5341 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using LysRS Antibody.

LysRS (A-10) - sc-271331 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LysRS (A-10): sc-271331. Western blot analysis of LysRS expression in HeLa (A) and Jurkat (B) whole cell lysates.

LysRS (B-2) - sc-393180 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LysRS (B-2): sc-393180. Western blot analysis of LysRS expression in HeLa (A), Caki-1 (B) and PC-3 (C) whole cell lysates.

LysRS (D-4) - sc-393645 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LysRS (D-4): sc-393645. Western blot analysis of LysRS expression in HeLa (A), Jurkat (B), Caki-1 (C) and COLO 320DM (D) whole cell lysates.

LysRS (Y-16) - sc-79256 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LysRS (Y-16): sc-79256. Western blot analysis of LysRS expression in HeLa whole cell lysate.

LysRS (H-300) - sc-98559 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LysRS (H-300): sc-98559. Western blot analysis of LysRS expression in non-transfected 293T: sc-117752 (A), mouse LysRS transfected 293T: sc-121459 (B) and NIH/3T3 (C) whole cell lysates.

LysRS (C-18) - sc-79253 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LysRS (C-18): sc-79253. Western blot analysis of LysRS expression in non-transfected: sc-117752 (A) and mouse LysRS transfected: sc-121459 (B) 293T whole cell lysates.

Anti-Lysyl tRNA synthetase antibody - ab31532 from Abcam

Prices $380.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab31532 staining Lysyl tRNA synthetase in normal human breast tissue by Immunohistochemistry (paraffin embedded sections).

Anti-Lysyl tRNA synthetase antibody - ab155114 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Lysyl tRNA synthetase antibody (ab155114) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

Anti-Lysyl tRNA synthetase antibody - ab70524 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Lysyl tRNA synthetase with ab70524 at 1/200 (1µg/ml). Detection: DAB.

Anti-Lysyl tRNA synthetase antibody - ab91595 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab91595, at 1/100 dilution, staining Lysyl tRNA synthetase in formalin-fixed paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry using Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 secondary antibody at a dilution of 1/100

Anti-Lysyl tRNA synthetase antibody [8G12C1] - ab70427 from Abcam

Host Mouse
Clonality Monoclonal
Clone 8G12C1
ab70427 staining Lysil tRNA synthetase in human cervical carcinoma by Immunohistochemsitry, Paraffin-embedded tissue.

Anti-Lysyl tRNA synthetase antibody [EPR7920] - ab134912 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7920
All lanes : Anti-Lysyl tRNA synthetase antibody [EPR7920] (ab134912) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : HepG2 cell lysateLane 5 : Caco 2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Anti-Lysyl tRNA synthetase antibody [EPR7921] - ab129080 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7921
ICC/IF image of ab129080 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129080 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-KARS Antibody (Internal, clone EPR7921) - LS-C152578 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7921
IHC of paraffin-embedded human colon tissue using anti-KARS antibody.

Anti-KARS Antibody (N-Terminus, clone EPR7920) - LS-C154677 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7920
IHC of paraffin-embedded human breast carcinoma tissue using anti-KARS antibody.

Anti-KARS Antibody (C-Terminus) - LS-C286688 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Anti-KARS Antibody (aa70-98) - LS-C165398 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of KARS (arrow) using rabbit polyclonal KARS Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.

Anti-KARS Antibody (C-Terminus) IHC-plus™ - LS-B8077 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8077 dilution 5-10 ug/ml.

Anti-KARS Antibody (clone 8G12C1) IHC-plus™ - LS-B4096 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 8G12C1
Anti-KARS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.

Anti-KARS Antibody (N-Terminus) - LS-C47039 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-KARS Antibody (C-Terminus) - LS-C71258 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-KARS Antibody - LS-C190970 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KARS Antibody (C-Terminus) - LS-C287504 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1 Detection: DAB.
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