Anti-LYVE1 antibody - Lymphatic Vessel Marker
| Name | Anti-LYVE1 antibody - Lymphatic Vessel Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab10278 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-P WB |
| Species Reactivities | Human, Rat |
| Antigen | Recombinant fragment corresponding to Human LYVE1 aa 24-232 |
| Description | Rabbit Polyclonal |
| Gene | LYVE1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Frozen sections) analysis of human colon carcinoma tissue sections labelling LYVE1 with ab10278.
ab10278 (2µg/ml) staining LYVE1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is lymphatic endothelium staining of lymphatic ducts where blood vessel endothelium and smooth muscle is wholly negative.Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
All lanes : Anti-LYVE1 antibody - Lymphatic Vessel Marker (ab10278) at 1 µg/mlLane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab10278.
All lanes : Anti-LYVE1 antibody - Lymphatic Vessel Marker (ab10278)Lane 1 : Human LYVE1 protein fragment (ab54341)Lane 2 : Mouse LYVE1 protein fragment (ab54342)
Rat skin was fixed with paraformaldehyde in 15% saturated picric acid solution for 4hr. Prior to sectioning, the specimen was infiltrated in O.C.T. and frozen in isopentane. The frozen specimen was sectioned these were rinsed in PBS for 15 min to remove O.C.T. and incubated in a 3% sodium deoxycholate solution. The specimens were rinsed twice with distilled water and then with PBS three times. The sections were incubated in 10% normal goat serum for 12 hr at 4°C, then for 12 hr with ab10278. After washing with PBS, the specimens were incubated with Alexa Fluor® 555-conjugated goat anti-rabbit IgG (H+L) (1:500), for 12 hr at 4°C. The cell nuclei were counterstained with YoYo-1. Images were obtained by using confocal microscope.See Abreview
Product References
Nestin expression in pterygia: potential role in angiogenesis. - Nestin expression in pterygia: potential role in angiogenesis.
Tonthat CV, Di Girolamo N. Br J Ophthalmol. 2014 Jun;98(6):801-7.
Collagen-binding proteoglycan fibromodulin can determine stroma matrix structure - Collagen-binding proteoglycan fibromodulin can determine stroma matrix structure
Oldberg A, Kalamajski S, Salnikov AV, Stuhr L, Morgelin M, Reed RK, Heldin NE, Rubin K. Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):13966-71. Epub 2007 Aug 22.
Human breast microvascular endothelial cells retain phenotypic traits in - Human breast microvascular endothelial cells retain phenotypic traits in
Sigurdsson V, Fridriksdottir AJ, Kjartansson J, Jonasson JG, Steinarsdottir M, Petersen OW, Ogmundsdottir HM, Gudjonsson T. In Vitro Cell Dev Biol Anim. 2006 Nov-Dec;42(10):332-40.