Anti-M6PR (cation independent) antibody [MEM-238]
| Name | Anti-M6PR (cation independent) antibody [MEM-238] |
|---|---|
| Supplier | Abcam |
| Catalog | ab8093 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | MEM-238 |
| Applications | FC IP WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Primate |
| Antigen | Vaccinia-M6PR/iGFIIR |
| Description | Mouse Monoclonal |
| Gene | IGF2R |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab8093 at 5µg/ml staining human HEK cells by immunocytochemistry. The cells were fixed with paraformaldehyde and incubated with the antibody for 1 hour. Bound antibody was detected using a goat anti-mouse IgG Alexa-Fluor ® 568. In the confocal image ab8093 labelling in red shows a distribution consistent with the location of the trans-Golgi network and lysosomes. Blue nuclear counterstain is present. This image is courtesy of an Abreview submitted by Randal Moldrich on 31 March 2006.See Abreview
ab8093 staining Mannose 6 Phosphate Receptor (Cation independent) in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.1% Triton X-100 and blocked with 2% BSA for 30 minutes at 25°C . Samples were incubated with primary antibody (1/300 in 2%BSA + 0.1% Triton X-100) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG (dilution 1/2000) was used as secondary antibody.See Abreview
![Overlay histogram showing HeLa cells stained with ab8093 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8093, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/18351_Mannose-6-Phosphate-Receptor-Cation-independent-Primary-antibodies-ab8093-5.jpg)
Overlay histogram showing HeLa cells stained with ab8093 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8093, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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