Anti-M6PR (cation independent) antibody [MEM-238] (FITC)
| Name | Anti-M6PR (cation independent) antibody [MEM-238] (FITC) |
|---|---|
| Supplier | Abcam |
| Catalog | ab58841 |
| Prices | $193.00 |
| Sizes | 50 tests |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | MEM-238 |
| Applications | ICC/IF ICC/IF WB FC |
| Species Reactivities | Human, Primate |
| Antigen | Recombinant Vaccinia virus encoding Mannose 6 Phosphate Receptor (Cation independent) (CD222) |
| Description | Mouse Monoclonal |
| Gene | IGF2R |
| Conjugate | FITC |
| Supplier Page | Shop |
Product images
ab58841 staining Mannose 6 Phosphate Receptor in a Human cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% NP-40 + 1% BSA in PBS and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/50 in 0.5% NP-40 + 1% BSA in PBS) for 1 hour at 25°C.See Abreview
![Lane 1 : Anti-M6PR (cation independent) antibody [MEM-238] (FITC) (ab58841)Lane 2 : Mouse IgG1 isotype controlLane 1 : Jurkat whole cell lysateLane 2 : Jurkat whole cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/18353_ab58841wb.gif)
Lane 1 : Anti-M6PR (cation independent) antibody [MEM-238] (FITC) (ab58841)Lane 2 : Mouse IgG1 isotype controlLane 1 : Jurkat whole cell lysateLane 2 : Jurkat whole cell lysate
Overlay histogram showing HeLa cells stained with ab58841 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58841, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma - Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma
Laube F. Anticancer Res. 2009 Apr;29(4):1383-8.
The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in - The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in
Leksa V, Godar S, Cebecauer M, Hilgert I, Breuss J, Weidle UH, Horejsi V, Binder BR, Stockinger H. J Biol Chem. 2002 Oct 25;277(43):40575-82. Epub 2002 Aug 19.