Lane 1 : Anti-MACC1 antibody (ab106579) at 1 µg/mlLane 2 : Anti-MACC1 antibody (ab106579) at 2 µg/mlLane 1 : Mouse liver tissue lysateLane 2 : Mouse liver tissue lysateLysates/proteins at 15 µg per lane.
Immunohistochemical analysis of MACC1 expression in paraffin embedded Human liver tissue, using ab106579 at 2.5 µg/ml.
ICC/IF image of ab106579 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106579, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of MACC1 in Human Liver cells using ab106579 at 20 ug/ml.