Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling macroH2A.1 with ab183041 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). Counter stained with Dapi (blue).
Immunofluorescent analysis of acetone-fixed HeLa cells labeling macroH2A.1 with ab183041 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). Counter stained with Dapi (blue).
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling macroH2A.1 with ab183041 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling macroH2A.1 with ab183041 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-macroH2A.1 antibody [EPR9359(2)] (ab183041) at 1/50000 dilutionLane 1 : HepG2 cell lysateLane 2 : MCF7 cell lysateLane 3 : HeLa cell lysateLane 4 : 293T cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution