H2AFY

Gene Symbol H2AFY
Entrez Gene 9555
Alt Symbol H2A.y, H2A/y, H2AF12M, H2AFJ, MACROH2A1.1, mH2A1, macroH2A1.2
Species Human
Gene Type protein-coding
Description H2A histone family, member Y
Other Description core histone macro-H2A.1|histone H2A.y|histone macroH2A1|histone macroH2A1.1|histone macroH2A1.2|medulloblastoma antigen MU-MB-50.205
Swissprots Q96D41 O75367 Q7Z5E3 Q9UP96 Q9H8P3 Q503A8 O75377
Accessions EAW62218 EAW62219 EAW62220 EAW62221 EAW62222 O75367 AB209490 BAD92727 AF041483 AAC33433 AF044286 AAC33434 AF054174 AAC39908 AK023409 BAB14565 AK023833 AK092944 AK123153 AK296014 BAG58785 AY134746 AAN08620 BC013331 AAH13331 BC045570 BC063638 BC095406 AAH95406 BE262597 EU446955 ABZ92484 JF432887 ADZ16104 XM_005272132 XP_005272189 XM_005272134 XP_005272191 XM_005272135 XP_005272192 XM_011543728 XP_011542030 XM_011543729 XP_011542031 XM_011543730 XP_011542032 XM_011543731 XP_011542033 XM_011543732 XP_011542034 XM_011543733 XP_011542035 XM_011543734 XP_011542036 XM_011543735 XP_011542037 XR_948306 XR_948307 XR_948308 XR_948309 XR_948310 XR_948311 NM_001040158 NP_001035248 NM_004893 NP_004884 NM_138609 NP_613075 NM_138610 NP_613258
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP- ribose, and may be involved in ADP-ribose-mediated chromatin modulation. {ECO:0000269|PubMed:12718888, ECO:0000269|PubMed:15
Subcellular Location Nucleus. Chromosome. Note=Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:9714746}.
Top Pathways Systemic lupus erythematosus, Alcoholism

MacroH2A1 Antibody - 8551 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using MacroH2A1 Antibody.

MacroH2A1.1 (D5F6N) Rabbit mAb - 12455 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5F6N
Western blot analysis of extracts from various tissues using MacroH2A1.1 (D5F6N) Rabbit mAb.

MacroH2A1.2 Antibody - 4827 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, H-4-II-E and COS cells using MacroH2A1.2 Antibody.

MacroH2A1.1 Antibody - 4160 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells transfected with a MacroH2A1.1 expression construct, mouse kidney, mouse liver and human kidney using MacroH2A1.1 Antibody.

MacroH2A1 (I-11) - sc-161812 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MacroH2A1 (I-11): sc-161812. Western blot analysis of MacroH2A1 expression in Jurkat nuclear extract.

MacroH2A1 (K-14) - sc-161813 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-macroH2A.1 antibody - ab83797 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-macroH2A.1 antibody (ab83797) at 1 µg/ml + MCF7 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-macroH2A.1 antibody - ab83782 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-macroH2A.1 antibody (ab83782) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-macroH2A.1 antibody [14G7] - ab91528 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 14G7
All lanes : Anti-macroH2A.1 antibody [14G7] (ab91528) at 10 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-macroH2A.1 antibody [EPR9358] - ab137117 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9358
All lanes : Anti-macroH2A.1 antibody [EPR9358] (ab137117) at 1/1000 dilutionLane 1 : fetal kidney cell lysateLane 2 : HepG2 cell lysateLane 3 : HeLa cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-macroH2A.1 antibody [EPR9359(2)] - ab183041 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9359(2)
Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling macroH2A.1 with ab183041 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). Counter stained with Dapi (blue).

Anti-macroH2A.1 antibody - ChIP Grade - ab37264 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab37264 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab37264, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa and HEK 293 cells. 

Anti-H2AFY / MACROH2A1 Antibody (aa144-193) IHC-plus™ - LS-B10333 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-H2AFY / MACROH2A1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10333 concentration 5 ug/ml.

Anti-H2AFY / MACROH2A1 Antibody - LS-C129794 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-H2AFY / MACROH2A1 Antibody - LS-C129795 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-H2AFY / MACROH2A1 Antibody - LS-C155116 from LifeSpan Bioscience

Prices $245.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-H2AFY / MACROH2A1 Antibody (aa109-369, Biotin) - LS-C297483 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-H2AFY / MACROH2A1 Antibody (aa109-369, FITC) - LS-C304875 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-H2AFY / MACROH2A1 Antibody (N-Terminus) - LS-C108069 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
H2AFY antibody LS-C108069 Western blot of NCI-H226 cell lysate.

Anti-H2AFY / MACROH2A1 Antibody (aa36-85) - LS-C102764 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
H2AFY antibody LS-C102764 Western blot of MCF7 cell lysate.

Anti-H2AFY / MACROH2A1 Antibody (aa109-369) - LS-C294374 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant H2AFY / MACROH2A1.

Anti-H2AFY / MACROH2A1 Antibody - LS-C346387 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence blot of U20S cell using H2AFY antibody. Blue: DAPI for nuclear staining.

Histone H2AY/macroH2A.1 Antibody (866416) - MAB8318 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 866416
Western blot shows lysates of MCF‑7 human breast cancer cell line, Hepa 1‑6 mouse hepatoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Histone H2AY Monoclonal Antibody (Catalog # MAB8318) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone H2AY at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Histone H2AY/macroH2A.1 Antibody - NBP1-53018 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Histone H2AY/macroH2A.1 Antibody [NBP1-53018] - NCI-H226 cell lysate, concentration 0.2-1 ug/ml.

Histone H2AY/macroH2A.1 Antibody - NBP1-53003 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Histone H2AY/macroH2A.1 Antibody [NBP1-53003] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.
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