Anti-macroH2A.1 antibody - ChIP Grade
| Name | Anti-macroH2A.1 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab37264 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP FC WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rat, Chicken, Bovine, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human macroH2A |
| Description | Rabbit Polyclonal |
| Gene | H2AFY |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab37264 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab37264, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa and HEK 293 cells.
IHC image of macroH2A.1 staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37264, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
All lanes : Anti-macroH2A.1 antibody - ChIP Grade (ab37264) at 1 µgLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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MacroH2A histone variants act as a barrier upon reprogramming towards - MacroH2A histone variants act as a barrier upon reprogramming towards
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