Anti-MAD1 antibody [9B10]
| Name | Anti-MAD1 antibody [9B10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab5783 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 9B10 |
| Applications | IP ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length MAD1 protein (Human) |
| Description | Mouse Monoclonal |
| Gene | MAD1L1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab5783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5783, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/18484_ab5783-2-ab5783FC.jpg)
Overlay histogram showing HeLa cells stained with ab5783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5783, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
Phosphorylation of nucleoporin Tpr governs its differential localization and is - Phosphorylation of nucleoporin Tpr governs its differential localization and is
Rajanala K, Sarkar A, Jhingan GD, Priyadarshini R, Jalan M, Sengupta S, Nandicoori VK. J Cell Sci. 2014 Aug 15;127(Pt 16):3505-20.
Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the - Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the
Hewitt L, Tighe A, Santaguida S, White AM, Jones CD, Musacchio A, Green S, Taylor SS. J Cell Biol. 2010 Jul 12;190(1):25-34.