MAD1L1

Gene Symbol MAD1L1
Entrez Gene 8379
Alt Symbol MAD1, PIG9, TP53I9, TXBP181
Species Human
Gene Type protein-coding
Description MAD1 mitotic arrest deficient-like 1 (yeast)
Other Description MAD1-like protein 1|mitotic arrest deficient 1-like protein 1|mitotic checkpoint MAD1 protein homolog|mitotic spindle assembly checkpoint protein MAD1|mitotic-arrest deficient 1, yeast, homolog-like 1|tax-binding protein 181|tumor protein p53 inducible protein 9
Swissprots Q9UNH0 Q9Y6D9 Q86UM4 Q13312 B3KR41 Q75MI0
Accessions AAP21876 AAS07503 EAL23947 EAW87219 EAW87220 Q9Y6D9 AF083811 AAD24498 AF123318 AAD20359 AK090959 BAG52253 AK310481 AK310831 AK314227 BC009964 AAH09964 BC018870 BG762406 BM818088 BQ684437 BQ687750 BU617803 EU831486 ACE87173 EU831573 ACE86492 U33822 AAC52059 XM_005249877 XP_005249934 XM_011515567 XP_011513869 XM_011515568 XP_011513870 XM_011515569 XP_011513871 XM_011515570 XP_011513872 XM_011515571 XP_011513873 NM_001013836 NP_001013858 NM_001013837 NP_001013859 NM_001304523 NP_001291452 NM_001304524 NP_001291453 NM_001304525 NP_001291454 NM_003550 NP_003541
Function Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. {ECO:0000269|PubMed:10049595, ECO:0000269|PubMed:12837246, ECO:0000269|PubMed:20133940}.
Subcellular Location Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
Tissue Specificity Expressed weakly at G0/G1 and highly at late S and G2/M phase.
Top Pathways Cell cycle, Progesterone-mediated oocyte maturation, Viral carcinogenesis

MAD1 (F-7) - sc-376613 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD1 (F-7): sc-376613. Western blot analysis of MAD1 expression in non-transfected 293T: sc-117752 (A), human MAD1 transfected 293T: sc-177504 (B) and SK-N-SH (C) whole cell lysates.

MAD1 (117-470) - sc-65493 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD1 (117-470): sc-65493. Western blot analysis of MAD1 expression in HeLa (A) and A-431 (B) whole cell lysates.

MAD1 (117-468) - sc-65494 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

MAD1 (D-1) - sc-166312 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD1 (D-1): sc-166312. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

MAD1 (D-5) - sc-166313 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD1 (D-5): sc-166313. Western blot analysis of MAD1 expression in non-transfected 293T: sc-117752 (A), mouse MAD1 transfected 293T: sc-121481 (B) and HeLa (C) whole cell lysates.

MAD1 (D-6) - sc-137026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD1 (D-6): sc-137026. Western blot analysis of MAD1 expression in non-transfected: sc-117752 (A) and mouse MAD1 transfected: sc-121481 (B) 293T whole cell lysates.

MAD1 (G-12) - sc-166322 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD1 (G-12): sc-166322. Western blot analysis of MAD1 expression in SK-N-SH (A) and HeLa (B) whole cell lysates.

MAD1 (L-17) - sc-54962 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MAD1 (L-17) : sc-54962. Western blot analysis of MAD1 expression in HeLa (A), U-251-MG (B) and U-698-M (C) whole cell lysates and HeLa nuclear extract (D).

MAD1 (H-228) - sc-67338 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MAD1 (H-228): sc-67338. Western blot analysis of MAD1 expression in A-431 (A) and HeLA (B) whole cell lysates and HeLa nuclear extract (C).

MAD1 (C-5) - sc-137025 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD1 (C-5): sc-137025. Western blot analysis of MAD1 expression in non-transfected: sc-117752 (A) and mouse MAD1 transfected: sc-121481 (B) 293T whole cell lysates.

MAD1 (M-300) - sc-67337 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MAD1 (M-300): sc-67337. Western blot analysis of MAD1 expression in non-transfected: sc-117752 (A) and mouse MAD1 transfected: sc-121481 (B) 293T whole cell lysates.

MAD1 (N-20) - sc-54963 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MAD1 (N-20): sc-54963. Western blot analysis of MAD1 expression in non-transfected 293T: sc-117752 (A), human MAD1 transfected 293T: sc-177505 (B) and HeLa (C) whole cell lysates.

MAD1 (9B10) - sc-47746 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD1 (9B10): sc-47746. Western blot analysis of MAD1 expression in HeLa (A), A-431 (B), BJAB (C) and Meg-01 (D) nuclear extracts.Replacement for gel in Book 140-51.

Anti-MAD1 antibody - ab175245 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling MAD1 with ab175245 at 1/200. Magnification: 400x.

Anti-MAD1 antibody - ab126148 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-MAD1 antibody (ab126148) at 1/1000 dilution + MCF-7 whole cell lysate at 30 µg

Anti-MAD1 antibody - ab155236 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft labeling MAD1 using ab155236 at 1/100 dilution.

Anti-MAD1 antibody - ab84490 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC analysis of FFPE section of human prostate adenocarcinoma using ab84490 at a dilution of 1/250.

Anti-MAD1 antibody [9B10] - ab5783 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9B10
Overlay histogram showing HeLa cells stained with ab5783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5783, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-MAD1 antibody [EPR14676] - ab184175 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14676
All lanes : Anti-MAD1 antibody [EPR14676] (ab184175) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : MCF7 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-MAD1 antibody [EPR14676-23] - ab184560 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14676-23
Immunohistochemical analysis of formalin fixed and paraffin embedded Human melanoma tissue labeling MAD1  with ab184560 at 1/50 dilution, counterstained with Hematoxylin.

Anti-MAD1 antibody [MAD1D6H4] - ab50791 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MAD1D6H4
Anti-MAD1 antibody [MAD1D6H4] (ab50791) + Immunised recombinant protein

Anti-MAD1L1 / MAD1 Antibody (aa1-350) - LS-C37274 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-MAD1L1 / MAD1 Antibody - LS-C18971 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-MAD1L1 / MAD1 Antibody (C-Terminus) - LS-C16303 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MAD1L1 / MAD1 Antibody (aa1-50) - LS-C71972 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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