All lanes : Anti-Mad2L1 antibody (ab97777) at 1/1000 dilutionLane 1 : HepG2 whole cell lysateLane 2 : MOLT4 whole cell lysateLane 3 : Raji whole cell lysateLysates/proteins at 30 µg per lane.
Immunofluorescence analysis of Mad2L1 in Human osteosarcoma cell line U2OS, using ab97777 at 1/1000 dilution. 1: Mad2L1 2: ACA 3: merged with DAPI Cells were fixed with 4% PFA/PBS for 15min, RT and blocked with 2.5% BSA/PBS.
Immunohistochemical analysis of Mad2L1 in paraffin embedded DLD-1 xenograft, using ab97777 at a 1/500 dilution.