MAD2L1

Gene Symbol MAD2L1
Entrez Gene 4085
Alt Symbol HSMAD2, MAD2
Species Human
Gene Type protein-coding
Description MAD2 mitotic arrest deficient-like 1 (yeast)
Other Description MAD2 (mitotic arrest deficient, yeast, homolog)-like 1|MAD2-like protein 1|mitotic arrest deficient 2-like protein 1|mitotic arrest deficient, yeast, homolog-like 1|mitotic spindle assembly checkpoint protein MAD2A
Swissprots Q6IRW7 Q548X9 Q53F56 Q13257 Q8IZX3
Accessions AAK38174 AAY40945 BAB63410 EAX05270 EAX05271 EAX05273 Q13257 AA443733 AF394735 AAN74648 AJ000186 CAA03943 AK223433 BAD97153 AK298228 BAG60497 AK313827 BAG36562 BC000356 AAH00356 BC005945 AAH05945 BC070283 AAH70283 EU831987 ACE87428 EU832080 ACE86742 NM_002358 NP_002349
Function Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore- spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. {ECO:0000269|PubMed:10700282, ECO:0000269|PubMed:11804586, ECO:0000269|PubMed:15024386}.
Subcellular Location Nucleus. Chromosome, centromere, kinetochore. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Note=Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. {ECO:0000305}.
Top Pathways Cell cycle, Progesterone-mediated oocyte maturation, Oocyte meiosis, HTLV-I infection

MAD2L1 (D8A7) Rabbit mAb - 4636 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8A7
Western blot analysis of extracts from various cell lines using MAD2L1 (D8A7) Rabbit mAb.

MAD2 (17D10) - sc-47747 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD2 (17D10): sc-47747. Western blot analysis of MAD2 expression in Jurkat (A), HeLa (B) and MEG-01 (C) whole cell lysates and Jurkat (D), BJAB (E) and HeLa (F) nuclear extracts.

MAD2 (C-9) - sc-393188 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD2 (C-9): sc-393188. Western blot analysis of MAD2 expression in Jurkat (A), K-562 (B), HeLa (C), BJAB (D) and MEG-01 (E) nuclear extracts.

MAD2 (C-10) - sc-374131 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD2 (C-10): sc-374131. Western blot analysis of MAD2 expression in Jurkat (A) and MEG-01 (B) nuclear extracts.

MAD2 (C-19) - sc-6329 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MAD2 (C-19): sc-6329. Western blot analysis of MAD2 expression in NAMALWA (A), K-562 (B), Jurkat (C) and HeLa (D) nuclear extracts.

MAD2 (E-17) - sc-31790 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MAD2 (E-17): sc-31790. Immunofluorescence staining of methanol-fixed BJAB cells showing nuclear localization.

MAD2 (N-19) - sc-6330 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MAD2 (N-19): sc-6330. Western blot analysis of MAD2 expression in BJAB (A), K-562 (B) and Jurkat (C) nuclear extracts.

MAD2 (S-20) - sc-31788 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MAD2 (S-20): sc-31788. Western blot analysis of MAD2 expression in non-transfected: sc-117752 (A) and human MAD2 transfected: sc-177506 (B) 293T whole cell lysates.

MAD2 (FL-205) - sc-28261 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MAD2 (FL-205): sc-28261. Western blot analysis of MAD2 expression in HeLa (A), BJAB (B), MEG-01 (C) and Jurkat (D) nuclear extracts.

MAD2 (107-276-3) - sc-65492 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-MAD2 antibody - ab189654 from Abcam

Prices $407.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-MAD2 antibody (ab189654) at 1/1000 dilution + 293T whole cell lysate at 30 µg

Anti-MAD2 antibody - ab61591 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MAD2 antibody (ab61591) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-Mad2L1 antibody - ab24588 from Abcam

Prices $382.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab24588 at a 1/100 dilution staining human breast cancer cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% BSA prior to incubation with the antibody for 1 hour. A TRITC conjugated polyclonal anti-rabbit Ig was used as the secondary antibody. The image shows staining with ab24588 as well as a DNA stain. The right hand panel shows a merged image.See Abreview

Anti-Mad2L1 antibody - ab97777 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Mad2L1 antibody (ab97777) at 1/1000 dilutionLane 1 : HepG2 whole cell lysateLane 2 : MOLT4 whole cell lysateLane 3 : Raji whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Mad2L1 antibody - ab97776 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Mad2L1 antibody (ab97776) at 1/1000 dilution + 293T whole cell lysate at 30 µg

Anti-Mad2L1 antibody - ab3632 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Mad2L1 antibody (ab3632) at 1/1200 dilutionLane 1 : Jurkat whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : Jurkat whole cell lysate with immunising peptideLane 4 : HeLa whole cell lysate with immunising peptideLysates/proteins at 35 µg per lane.SecondaryIRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilutionPerformed under reducing conditions.

Anti-Mad2L1 antibody - ab55452 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Mad2L1 antibody - ab87274 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Mad2L1 by ab87274 at 1:250 dilution using a Formalin-Fixed Paraffin-Embedded section of human breast carcinoma. Detection by means of DAB staining.

Anti-Mad2L1 antibody [17D10] - ab10691 from Abcam

Prices $390.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 17D10
ab10691 staining Mad2L1 in colon cancer tissue, after antigen retreival, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Anti-Mad2L1 antibody [4D2] - ab114036 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4D2
All lanes : Anti-Mad2L1 antibody [4D2] (ab114036) at 1/1000 dilutionLane 1 : HEK293T cell lysate transfected with the pCMV6-ENTRY control cDNA Lane 2 : HEK293T cell lysate transfected with the pCMV6-ENTRY Mad2L1 cDNA Lysates/proteins at 5 µg per lane.

Anti-Mad2L1 antibody [EPR9852] - ab171084 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9852
All lanes : Anti-Mad2L1 antibody [EPR9852] (ab171084) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Jurkat cell lysateLane 3 : Raji cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.

Anti-Mad2L1 antibody [EPR9853(B)] - ab150371 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9853(B)
All lanes : Anti-Mad2L1 antibody [EPR9853(B)] (ab150371) at 1/10000 dilutionLane 1 : 293T cell lysatesLane 2 : Jurkat cell lysatesLane 3 : Raji cell lysatesLane 4 : A431 cell lysatesLysates/proteins at 10 µg per lane.

Anti-Mad2L1 antibody [GT1761] - ab184620 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone GT1761
All lanes : Anti-Mad2L1 antibody [GT1761] (ab184620) at 1/250 dilutionLane 1 : Jurkat whole cell lysate extractLane 2 : Raji whole cell lysate extractLysates/proteins at 30 µg per lane.

Anti-MAD2L1 / MAD2 Antibody - LS-C48689 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MAD2L1 / MAD2 Antibody (aa192-205) - LS-C55078 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
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