Immunoprecipitation of Hela cells labeling Mad2L2 using ab180579 at 1/40 dilution (lane 1) or negative control (lane 2) followed by anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
Flow cytometric analysis of paraformadehyde fixed K562 cells labeling Mad2L2 with ab180579 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/50.
Immunocytochemical staining of paraformaldehyde fixed Hela cells labeling Mad2L2 with ab180579 at 1/100 dilution and Goat anti rabbit IgG(Dylight 488) at 1/200 dilution. Counterstained with DAPI (blue).
Immunohistochemical staining of paraffin-embedded Human ovarian carcinoma labeling Mad2L2 at using ab180579 1/50 dilution and ImmunoHistoprobe HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
All lanes : Anti-Mad2L2 antibody [EPR13657] (ab180579) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : SW480 cell lysateLane 3 : Hela cell lysateLane 4 : Molt-4 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution