MAD2L2

Gene Symbol MAD2L2
Entrez Gene 10459
Alt Symbol MAD2B, POLZ2, REV7
Species Human
Gene Type protein-coding
Description MAD2 mitotic arrest deficient-like 2 (yeast)
Other Description MAD2 (mitotic arrest deficient, yeast, homolog)-like 2|MAD2-like protein 2|REV7 homolog|hREV7|mitotic arrest deficient 2-like protein 2|mitotic arrest deficient homolog-like 2|mitotic spindle assembly checkpoint protein MAD2B|polymerase (DNA-directed), zeta 2, accessory subunit
Swissprots Q9UNA7 Q9Y6I6 Q5TGW7 B3KNE3 Q9UI95
Accessions AAY26393 EAW71697 EAW71698 EAW71699 Q9UI95 AF072933 AAD41647 AF080398 AAF20267 AF139365 AAD30290 AF157482 AAF34357 AK027327 BAG51305 AK094316 BAG52858 BC015244 AAH15244 BI517654 DQ891038 ABM81964 DQ894217 ABM85143 XM_011540507 XP_011538809 NM_001127325 NP_001120797 NM_006341 NP_006332
Function Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. {ECO:0000269|PubMed:11459825, ECO:0000269|PubMed:11459826, ECO:0000269|PubMed:17296730, ECO:0000269|PubMed:17719540, ECO:00
Subcellular Location Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm.
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:11717438}.
Top Pathways Cell cycle, Progesterone-mediated oocyte maturation, Oocyte meiosis, Bacterial invasion of epithelial cells

MAD2B (C-14) - sc-27731 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MAD2B (C-14): sc-27731. Western blot analysis of MAD2B expression in non-transfected: sc-110760 (A) and human MAD2B transfected: sc-113252 (B) 293 whole cell lysates.

MAD2B (H-82) - sc-292425 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

MAD2B (T-18) - sc-27730 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MAD2B (F-12) - sc-377367 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAD2B (F-12): sc-377367. Western blot analysis of MAD2B expression in non-transfected: sc-110760 (A) and human MAD2B transfected: sc-113252 (B) 293 whole cell lysates.

MAD2B (14) - sc-135977 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
MAD2B (14): sc-135977. Western blot analysis of MAD2B expression in K-562 whole cell lysate.

Anti-Mad2L2 antibody - ab115622 from Abcam

Prices $394.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Mad2L2 antibody (ab115622) at 1/1000 dilutionLane 1 : Mouse E10.5 whole embryo lysateLane 2 : Mouse E12.5 whole embryo lysateLane 3 : Mouse E17.5 whole brain lysateLane 4 : HeLa cell lysateSecondaryHRP-conjugated Goat anti-rabbit IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Mad2L2 antibody - ab168660 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-Mad2L2 antibody (ab168660) at 1 µg/mlLane 1 : MAD2L2 transfected 293T lysate.Lane 2 : Non-transfected 293T lysate.Lysates/proteins at 15 µl per lane.

Anti-Mad2L2 antibody - ab3630 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Mad2L2 antibody (ab3630) at 1/500 dilutionLane 1 : Whole cell extract prepared from Hela cellsLane 2 : Nuclear lysates prepared from Hela cellsLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Anti-Mad2L2 antibody [EPR13657] - ab180579 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13657
Immunoprecipitation of Hela cells labeling Mad2L2 using ab180579 at 1/40 dilution (lane 1) or negative control (lane 2) followed by anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.  

Anti-MAD2B / REV7 Antibody - LS-C309193 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-MAD2B / REV7 Antibody - LS-C311344 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MAD2B / REV7 Antibody - LS-C311346 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MAD2B / REV7 Antibody (aa3-14) IHC-plus™ - LS-B390 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MAD2L2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B390 concentration 10 ug/ml.

Anti-MAD2B / REV7 Antibody (aa198-231) - LS-C344613 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical of paraffin-embedded H. kidney section using MAD2L2 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

MAD2L2 Antibody - H00010459-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: MAD2L2 Antibody [H00010459-D01P] - Analysis of MAD2L2 expression in transfected 293T cell line by MAD2L2 polyclonal antibody.Lane 1: MAD2L2 transfected lysate(24.30 KDa).Lane 2: Non-transfected lysate.

MAD2L2 Antibody - NBP1-02798 from Novus Biologicals

Prices $329.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western Blot: MAD2L2 Antibody [NBP1-02798] - Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.

MAD2L2 Antibody - H00010459-B02 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: MAD2L2 Antibody [H00010459-B02] - analysis of MAD2L2 expression in transfected 293T cell line by MAD2L2 MaxPab polyclonal antibody (B02). Lane 1: MAD2L2 transfected lysate (24.30 KDa). Lane 2: Non-transfected lysate.

MAD2L2 Antibody - H00010459-B02P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: MAD2L2 Antibody [H00010459-B02P] - Analysis of MAD2L2 expression in transfected 293T cell line by MAD2L2 polyclonal antibody. Lane 1: MAD2L2 transfected lysate(23.21 KDa). Lane 2: Non-transfected lysate.

MAD2L2 Antibody - H00010459-D01 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Mad2L2 Polyclonal Antibody - bs-7840R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MAD2L2 antibody produced in rabbit - SAB2900339 from Sigma-Aldrich

Prices $464.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MAD2L2: <B>Cat. No.SAB2900339</B>: Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE).

Anti-MAD2L2 antibody produced in mouse - SAB1406994 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MAD2L2: <B>Cat. No. SAB1406994</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~23.2 kDa using MAD2L2 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-MAD2L2 (174-185) antibody produced in rabbit - SAB1100918 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-MAD2L2 (174-185) : <B>Cat. No. SAB1100918</B>: Immunofluorescence of HUVEC cells using MAD2L2 (174-185) , Cat. No. SAB1100918 (red) at a 1:100 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

MAD2L2-Antibody-C-term - AP20654c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded H. kidney section using MAD2L2 Antibody (C-term)(Cat#AP20654c). AP20654c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

MAD2L2-Antibody-C-term - AW5027 from Abgent, a WuXi AppTec company

Prices $110.00, $325.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of lysates from Hela, K562 cell line (from left to right), using MAD2L2 Antibody (C-term)(Cat. #AW5027). AW5027 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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