Anti-Mael antibody - C-terminal (ab175902) at 1/1000 dilution + HepG2 cell line lysate at 35 µg
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human testis tissue labeling Mael with ab175902 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow Cytometric analysis of HepG2 cells labeling Mael with ab175902 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-Mael antibody - C-terminal (ab175902) at 1/1000 dilutionLane 1 : 293 cell lysate, non-transfectedLane 2 : 293 cell lysate, transiently transfected with the Mael geneLysates/proteins at 2 µg per lane.