Anti-MafA antibody - ChIP Grade
| Name | Anti-MafA antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab17976 |
| Prices | $400.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ChIP EMSA ICC/IF ICC/IF IHC-P IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Mouse MafA (C terminal) |
| Description | Rabbit Polyclonal |
| Gene | MAFA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody.
Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069, anti-MafB Antibody or anti-c-Maf antibody.
ab17976 staining MAFA in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in blocking buffer) for 16 hours at 22°C. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.See Abreview
Detection of MafA, MafB and cMaf by Western Blot. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody, anti-MafB Antibody or anti-c-Maf antibody. Each antibody was used at 0.5 ug/ml. Detection: Chemiluminescence with a 5 second exposure.
Product References
VMAT2 identified as a regulator of late-stage beta-cell differentiation. - VMAT2 identified as a regulator of late-stage beta-cell differentiation.
Sakano D, Shiraki N, Kikawa K, Yamazoe T, Kataoka M, Umeda K, Araki K, Mao D, Matsumoto S, Nakagata N, Andersson O, Stainier D, Endo F, Kume K, Uesugi M, Kume S. Nat Chem Biol. 2014 Feb;10(2):141-8.
Identification and characterization of poorly differentiated invasive carcinomas - Identification and characterization of poorly differentiated invasive carcinomas
Hunter KE, Quick ML, Sadanandam A, Hanahan D, Joyce JA. PLoS One. 2013 May 14;8(5):e64472.
TAT-mediated transduction of MafA protein in utero results in enhanced pancreatic - TAT-mediated transduction of MafA protein in utero results in enhanced pancreatic
Vargas N, Alvarez-Cubela S, Giraldo JA, Nieto M, Fort NM, Cechin S, Garcia E, Espino-Grosso P, Fraker CA, Ricordi C, Inverardi L, Pastori RL, Dominguez-Bendala J. PLoS One. 2011;6(8):e22364.