MAFA

Gene Symbol MAFA
Entrez Gene 389692
Alt Symbol RIPE3b1, hMafA
Species Human
Gene Type protein-coding
Description v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A
Other Description pancreatic beta-cell-specific transcriptional activator|transcription factor MafA|transcription factor RIPE3b1
Swissprots Q8NHW3
Accessions AAL89527 BAC20389 BAJ04327 Q8NHW3 BC156443 AAI56444 NM_201589 NP_963883
Function Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. {ECO:0000269|PubMed:12011435, ECO:0000269|PubMed:12368292, ECO:0000269|PubMed:15665000, ECO:0000269|PubMed:15993959, ECO:0000269|PubMed:18042454, ECO:0000269|PubMed:19143053}.
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00978, ECO:0000269|PubMed:11416124, ECO:0000269|PubMed:12368292}. Note=Detected in nuclei of pancreas islet beta cells. {ECO:0000250}.
Top Pathways Type II diabetes mellitus, Maturity onset diabetes of the young

MafA (E-13) - sc-27138 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MafA (E-13): sc-27138. Western blot analysis of MafA expression in mouse eye (A) and mouse pancreas (B) tissue extracts.

MafA (F-6) - sc-390491 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MafA (F-6): sc-390491. Western blot analysis of MafA expression in mouse eye tissue extract.

MafA (H-38) - sc-66958 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

MafA (F-12) - sc-27140 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MafA (F-12): sc-27140. Western blot analysis of MafA expression in mouse eye (A) and mouse pancreas (B) tissue extracts.

MafA (H-12) - sc-27139 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-MAFA antibody - ab26405 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab26405 staining MAFA in Human PANC-1 cell line transfected with synthetic mRNA by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.05% Triton X-100 and blocked with 5% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200) for 30 minutes at 37°C. An Alexa Fluor® 555-conjugated donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.See Abreview

Anti-MAFA antibody - ab98859 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab98859 stained MCF7 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98859, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MAFA antibody - ab86020 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MAFA antibody (ab86020) at 1/1000 dilutionLane 1 : Uninduced (negative control) culture of E.coliLane 2 : Induced culture of E.coliSecondaryanti-Rabbit IgG HRP conjugated at 1/1000 dilution

Anti-MAFA antibody - ab124108 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-MAFA antibody (ab124108) at 1/100 dilution + K-562 cell line lysates at 35 µg

Anti-MAFA antibody - ab57807 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57807 MAFA antibody at 1ug/ml.

Anti-MafA antibody - ChIP Grade - ab17976 from Abcam

Prices $400.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody.

Anti-MAFA Antibody (C-Terminus) - LS-C71987 from LifeSpan Bioscience

Prices $635.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MAFA Antibody (aa286-315, APC) - LS-C237352 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAFA Antibody (aa286-315, FITC) - LS-C237353 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAFA Antibody (aa286-315, Biotin) - LS-C237354 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAFA Antibody (aa286-315, PE) - LS-C237355 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAFA Antibody (aa286-315, Azide-free, HRP) - LS-C248755 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAFA Antibody (aa286-315, AP) - LS-C249999 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAFA Antibody (aa300-350) - LS-C148555 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of MafA in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver lysate and 4) rat liver lysate using LS-C148555 at 0.25 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Anti-MAFA Antibody (C-Terminus) - LS-C116138 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MAFA antibody LS-C116138 Western blot of Mouse Muscle lysate.

Anti-MAFA Antibody (aa81-130) - LS-C116139 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MAFA antibody LS-C116139 Western blot of Mouse Kidney lysate.

Anti-MAFA Antibody (aa110-159) - LS-C81121 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-MAFA Antibody (aa71-120) - LS-C107898 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MAFA antibody LS-C107898 Western blot of Fetal Thymus lysate.

Anti-MAFA Antibody (C-Terminus) - LS-C287487 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MAFA Antibody (aa222-309) - LS-C308851 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
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