Anti-MALT1 antibody [EP603Y]

• Supplier: Abcam
• Catalog: ab33921
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP603Y
• Applications: WB FC
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues in the N term of MALT1 (Human)
• Description: Rabbit Monoclonal
• Gene: MALT1
• Conjugate: Unconjugated

Product images

Overlay histogram showing Jurkat cells stained with ab33921 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33921, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-MALT1 antibody [EP603Y] (ab33921) at 1/2000 dilution + Jurkat cell lysate

Product References

MALT1 auto-proteolysis is essential for NF-kappaB-dependent gene transcription in - MALT1 auto-proteolysis is essential for NF-kappaB-dependent gene transcription in

Baens M, Bonsignore L, Somers R, Vanderheydt C, Weeks SD, Gunnarsson J, Nilsson E, Roth RG, Thome M, Marynen P. PLoS One. 2014 Aug 8;9(8):e103774.

A quantitative signaling screen identifies CARD11 mutations in the CARD and LATCH - A quantitative signaling screen identifies CARD11 mutations in the CARD and LATCH

Chan W, Schaffer TB, Pomerantz JL. Mol Cell Biol. 2013 Jan;33(2):429-43.

Gene expression profiling of pulmonary mucosa-associated lymphoid tissue lymphoma - Gene expression profiling of pulmonary mucosa-associated lymphoid tissue lymphoma

Chng WJ, Remstein ED, Fonseca R, Bergsagel PL, Vrana JA, Kurtin PJ, Dogan A. Blood. 2009 Jan 15;113(3):635-45.

The protein kinase C-responsive inhibitory domain of CARD11 functions in - The protein kinase C-responsive inhibitory domain of CARD11 functions in

McCully RR, Pomerantz JL. Mol Cell Biol. 2008 Sep;28(18):5668-86.