Anti-Mannose Receptor antibody
| Name | Anti-Mannose Receptor antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab64693 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB IHC-F FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Chimpanzee, Monkey |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1400 to the C-terminus of Human Mannose Receptor |
| Description | Rabbit Polyclonal |
| Gene | MRC1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab64693 staining Mannose Receptor in Mouse heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 0.075% H2O2 for 10 minutes at room temperature. Samples were incubated with primary antibody (1/100 in 1x PBS + 10% heat inactivated FCS) for 1 hour. An undiluted HRP-conjugated Rabbit anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Anti-Mannose Receptor antibody (ab64693) at 1 µg/ml + Liver (Rat) Tissue Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab64693 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64693, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2, Hek293 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, MCF-7 cells at 5µg/ml.
IHC image of Mannose Receptor staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64693, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
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