All lanes : Anti-MAP3K12 antibody (ab37996) at 1/1000 dilutionLane 1 : HL-60 cell lysateLane 2 : SH-SY5Y cell lysateLysates/proteins at 35 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution
All lanes : Anti-MAP3K12 antibody (ab37996)Lane 1 : HL-60 cell lysateLane 2 : Mouse kidney tissue lysateSecondaryHRP-conjugated anti-rabbit IgGObserved band size : 95 kDa (why is the actual band size different from the predicted?)Additional bands at : 18 kDa,23 kDa,30 kDa,44 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MAP3K12 with ab37996. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.