All lanes : Anti-MAPK6 antibody [5E1] (ab155934) at 1/1000 dilutionLane 1 : HeLa whole cell lysateLane 2 : 293T whole cell lysateLane 3 : 3T3 whole cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse HRP conjugated antibody at 1/15000 dilutiondeveloped using the ECL technique
Anti-MAPK6 antibody [5E1] (ab155934) at 1/1000 dilution + HEK 293T cell overexpression lysate at 10 µgSecondaryGoat anti-mouse HRP conjugated antibody at 1/15000 dilution
Immunofluorescent analysis of formalin-fixed, permeabilized (0.1% Triton X-100) HT29 cells labeling MAPK6 with ab155934 at 1/100 (green, center image). The left hand image shows staining with Hoechst 33342 (blue); right image shows ab155934 and Hoechst 33342 staining merged.