Anti-MAPK6 antibody [EP1720Y]
| Name | Anti-MAPK6 antibody [EP1720Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab53277 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1720Y |
| Applications | IP WB FC IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues near the N-terminal of human MAPK6 |
| Description | Rabbit Monoclonal |
| Gene | MAPK6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing A431 cells stained with ab53277 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53277, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Anti-MAPK6 antibody [EP1720Y] (ab53277) at 1/1000 dilution + HeLa cell lysate at 10 µgSecondaryGoat anti-Rabbit HRP labeled at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/19468_ab53277_1.jpg)
Anti-MAPK6 antibody [EP1720Y] (ab53277) at 1/1000 dilution + HeLa cell lysate at 10 µgSecondaryGoat anti-Rabbit HRP labeled at 1/2000 dilution
Ab53277 (1:50) staining human MAPK6 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.Ab53277 (1:50) staining human MAPK6 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Product References
The non-classical MAP kinase ERK3 controls T cell activation. - The non-classical MAP kinase ERK3 controls T cell activation.
Marquis M, Boulet S, Mathien S, Rousseau J, Thebault P, Daudelin JF, Rooney J, Turgeon B, Beauchamp C, Meloche S, Labrecque N. PLoS One. 2014 Jan 27;9(1):e86681.
ERK3 signals through SRC-3 coactivator to promote human lung cancer cell - ERK3 signals through SRC-3 coactivator to promote human lung cancer cell
Long W, Foulds CE, Qin J, Liu J, Ding C, Lonard DM, Solis LM, Wistuba II, Qin J, Tsai SY, Tsai MJ, O'Malley BW. J Clin Invest. 2012 May;122(5):1869-80.
Activation loop phosphorylation of ERK3/ERK4 by group I p21-activated kinases - Activation loop phosphorylation of ERK3/ERK4 by group I p21-activated kinases
Deleris P, Trost M, Topisirovic I, Tanguay PL, Borden KL, Thibault P, Meloche S. J Biol Chem. 2011 Feb 25;286(8):6470-8.
Iron regulatory protein 2 turnover through a nonproteasomal pathway. - Iron regulatory protein 2 turnover through a nonproteasomal pathway.
Chang AH, Jeong J, Levine RL. J Biol Chem. 2011 Jul 8;286(27):23698-707.