ab63574, at 1/50 dilution, staining MAPKAP Kinase 2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
All lanes : Anti-MAPKAP Kinase 2 antibody (ab63574) at 1/500 dilutionLane 1 : HeLa cell extracts treated with Ca2+ (40nM, 30mins)Lane 2 : HeLa cell extracts treated with Ca2+ (40nM, 30mins) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.
All lanes : Anti-MAPKAP Kinase 2 antibody (ab63574) at 1/500 dilutionLane 1 : Human MAPKAP Kinase 2 full length protein (ab79910) at 0.01 µgLane 2 : Human MAPKAP Kinase 2 full length protein (ab79910) at 0.001 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 90 seconds