MAPKAPK2

Gene Symbol MAPKAPK2
Entrez Gene 9261
Alt Symbol MAPKAP-K2, MK-2, MK2
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase-activated protein kinase 2
Other Description MAP kinase-activated protein kinase 2|MAPK-activated protein kinase 2|MAPKAP kinase 2
Swissprots Q5SY30 Q8IYD6 Q5SY41 P49137
Accessions EAW93525 EAW93526 EAW93527 EAW93528 EAW93529 EAW93530 EAW93531 EAW93532 P49137 AK307499 BC036060 AAH36060 BC052584 AAH52584 BU621009 X75346 CAA53094 XM_005273353 XP_005273410 NM_004759 NP_004750 NM_032960 NP_116584
Function Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat- shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally
Subcellular Location Cytoplasm {ECO:0000269|PubMed:20932473}. Nucleus {ECO:0000269|PubMed:20932473}. Note=Phosphorylation and subsequent activation releases the autoinhibitory helix, resulting in the export from the nucleus into the cytoplasm.
Tissue Specificity Expressed in all tissues examined.
Top Pathways VEGF signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Viral carcinogenesis

Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 4320 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 27B7
Flow cytometric analysis of THP-1 cells, untreated (blue) or anisomycin-treated (green), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (Alexa Fluor® 647 Conjugate).

Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 4338 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 27B7
Flow cytometric analysis of THP1 cells, untreated (blue) or anisomycin-treated (green), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (Alexa Fluor® 488 Conjugate).

Phospho-MAPKAPK-2 (Thr334) Antibody - 3041 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from untreated or UV treated HeLa and Cos cells, using Phospho-MAPKAPK-2 (Thr334) Antibody (upper) or MAPKAPK-2 Antibody #3042 (lower).

Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb - 3316 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 9A7
Western blot analysis of extracts from HeLa cells, untreated (-) or UV- and TPA-treated (+), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower).

Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb - 3007 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 27B7
Western blot analysis of extracts from untreated or UV+TPA-treated HeLa and COS cells, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower).

MAPKAPK-2 (D1E11) Rabbit mAb - 12155 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1E11
Western blot analysis of extracts from various cell lines, untreated (-) or treated with UV (40 mJ/cm 2 , 30 min recovery; +), using MAPKAPK-2 (D1E11) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).

MAPKAPK-2 Antibody - 3042 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from untreated or UV treated HeLa and Cos cells using MAPKAPK-2 antibody.

Phospho-MAPKAPK-2 (Thr222) Antibody - 3044 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

MAPKAPK-2 (A-11) - sc-393609 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAPKAPK-2 (A-11): sc-393609. Western blot analysis of MAPKAPK-2 expression in Jurkat (A) and K-562 (B) whole cell lysates.

MAPKAPK-2 (C-18) - sc-6221 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MAPKAPK-2 (C-18): sc-6221. Western blot analysis of MAPKAPK-2 expression in A-431 whole cell lysate.

MAPKAPK-2 (H-66) - sc-7871 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MAPKAPK-2 (H-66): sc-7871. Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

MAPKAPK-2 (35-I) - sc-100393 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
MAPKAPK-2 (35-I): sc-100393. Western blot analysis of MAPKAPK-2 expression in K-562 whole cell lysate.

p-MAPKAPK-2 (Thr 222) - sc-31675 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-MAPKAPK-2 (Thr 222): sc-31675. Western blot analysis of MAPKAPK-2 phosphorylation in untreated (A), UV irradiated (B) and UV irradiated and lambda protein phosphatase treated (C) HeLa whole cell lysates.

p-MAPKAPK-2 (Thr 334) - sc-101729 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of phosphorylated MAPKAPK-2 expression in UV-treated HeLa whole cell lysates (A,B). Blots were probed with p-MAPKAPK-2 (Thr 334): sc-101729 preincubated with its cognate phosphorylated peptide (A) and p-MAPKAPK-2 (Thr 334): sc-101729 (B).

Anti-MAPKAP Kinase 2 (phospho T334) antibody - ab63378 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelled with ab63378 at 1/50 dilution. Right hand panel was treated with immunising phosphopeptide.

Anti-MAPKAP Kinase 2 (phospho T334) antibody - ab131504 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol-fixed HeLa cells labelling MAPKAP Kinase 2 (phospho T334) with ab131504 at 1/100 dilution.

Anti-MAPKAP Kinase 2 antibody - ab194452 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small Intestine tissue labeling MAPKAP Kinase 2 with ab194452 at 3 µg/ml.

Anti-MAPKAP Kinase 2 antibody - ab196759 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MAPKAP Kinase 2 antibody (ab196759) at 1/500 dilutionLane 1 : COS cell extractLane 2 : COS cell extract with synthesized peptide

Anti-MAPKAP Kinase 2 antibody - ab63574 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab63574, at 1/50 dilution, staining MAPKAP Kinase 2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.

Anti-MAPKAP Kinase 2 antibody [E341] - ab32567 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E341
Anti-MAPKAP Kinase 2 antibody [E341] (ab32567) at 1/1000 dilution + Hela cell lysate

Anti-MAPKAP Kinase 2 antibody [EPR5357] - ab133279 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5357
All lanes : Anti-MAPKAP Kinase 2 antibody [EPR5357] (ab133279) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : A431 cell lysateSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-MAPKAP Kinase 2 antibody - C-terminal - ab194410 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Immunocytochemistry/ Immunofluorescence of HeLa cells labeling MAPKAP Kinase 2 with ab194410 at 30 µg/mL.

Anti-MAPKAP Kinase 2 (phospho S272) antibody - ab138680 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MAPKAP Kinase 2 (phospho S272) antibody (ab138680) at 1/500 dilution + RAW 264.7 cell extracts, treated with UV (15 mins) at 30 µg

Anti-MAPKAP Kinase 2 (phospho S272) antibody - ab59998 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab59998 at 1/50 dilution staining MAPKAP Kinase 2 in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining

Anti-MAPKAP Kinase 2 (phospho T222) antibody - ab138652 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MAPKAP Kinase 2 (phospho T222) antibody (ab138652) at 1/500 dilution + COS7 cell extract (from cells treated with UV for 15 minutes) at 30 µg
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