All lanes : Anti-MAPKAP Kinase 3 antibody (ab64972) at 1/500 dilutionLane 1 : extracts from COLO205 cells, without immunising peptideLane 2 : extracts from COLO205 cells, with immunising peptide
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using MAPKAP Kinase 3 antibody (ab64972) at 1/50 dilution. Left: no immunising peptide. Right: with immunising peptide.
ICC/IF image of ab64972 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64972, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.