Immunoprecipitation analysis of Jurkat cells labeling MAPKAP Kinase 3 using ab183040 at 1/50 dilution. A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.
Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling MAPKAP Kinase 3 with ab183040 at 1/100 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink). Rabbit monoclonal IgG was used as Isotype control (green).
Anti-MAPKAP Kinase 3 antibody [EPR13969] (ab183040) at 1/5000 dilution + Jurkat cell line lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Anti-MAPKAP Kinase 3 antibody [EPR13969] (ab183040) at 1/20000 dilution + HeLa cell line lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution