All lanes : Anti-MASP3 antibody - Catalytic domain (ab65900) at 1/1000 dilutionLane 1 : Molecular weight markersLane 2 : 80ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 3 : 40ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 4 : 20ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 5 : 10ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 6 : 5ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Observed band size : 45 kDa (why is the actual band size different from the predicted?)