MASP1

Gene Symbol MASP1
Entrez Gene 5648
Alt Symbol 3MC1, CRARF, CRARF1, MAP1, MASP, MASP3, MAp44, PRSS5, RaRF
Species Human
Gene Type protein-coding
Description mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
Other Description Ra-reactive factor serine protease p100|complement factor MASP-3|complement-activating component of Ra-reactive factor|mannan-binding lectin serine protease 1|mannose-binding lectin-associated serine protease 1|mannose-binding protein-associated serine protease|serine protease 5
Swissprots Q9UF09 Q96RS4 Q68D21 Q8IUV8 P48740 O95570 A8K542 B4E2L7 A8K6M1
Accessions BAA34864 BAA89206 EAW78150 EAW78151 EAW78152 EAW78153 P48740 AF284421 AAK84071 AI278088 AI828640 AK129893 AK291157 BAF83846 AK291686 BAF84375 AK300663 BAG62349 AK304334 BAG65179 AL134380 AL137646 CAB70856 AL708781 AV686235 BC039724 AAH39724 BC106945 AAI06946 BC106946 AAI06947 BX641029 CAE46018 BX647237 CAI46037 CB162101 CR749615 CAH18409 DC423174 XM_006713700 XP_006713763 XM_006713701 XP_006713764 XM_011512989 XP_011511291 XM_011512990 XP_011511292 XM_011512991 XP_011511293 NM_001031849 NP_001027019 NM_001879 NP_001870 NM_139125 NP_624302 NR_033519
Function Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. {ECO:0000269|PubMed:11485744}.
Subcellular Location Secreted {ECO:0000269|PubMed:11485744}.
Tissue Specificity Protein of the plasma which is primarily expressed by liver. {ECO:0000269|PubMed:11485744, ECO:0000269|PubMed:8018603, ECO:0000269|PubMed:8240317, ECO:0000269|PubMed:9367419}.
Top Pathways Complement and coagulation cascades, Staphylococcus aureus infection

MASP-1 (C-20) - sc-50839 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MASP-1 (C-20): sc-50839. Western blot analysis of MASP-1 expression in mouse liver tissue extract.

MASP-1 (N-18) - sc-50841 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MASP-1 (N-18): sc-50841. Western blot analysis of MASP-1 expression in rat liver tissue extract.

MASP-1 (Q-20) - sc-50843 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MASP-1 (Q-20): sc-50843. Western blot analysis of MASP-1 expression in mouse liver tissue extract.

MASP-1 (W-19) - sc-50844 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MASP-1 (W-19): sc-50844. Western blot analysis of MASP-1 expression in rat liver tissue extract.

MASP-1/3 (F-12) - sc-166815 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MASP-1/3 (F-12): sc-166815. Western blot analysis of full-length human recombinant MASP-1.

MASP-1/3 (G-7) - sc-166816 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MASP-1/3 (G-7): sc-166816. Western blot analysis of full-length human recombinant MASP-1.

MASP-1/3 (H-260) - sc-48749 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MASP-1/3 (H-260): sc-48749. Western blot analysis of MASP-1/3 expression in rat testis (A), mouse uterus (B), rat liver (C) and mouse liver (D) tissue extracts.

MASP-1/3 (N-19) - sc-17904 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MASP-1/3 (N-20) - sc-17902 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MASP-3 (CD450) - sc-80177 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

Anti-MASP1/3 antibody - ab65888 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MASP1/3 antibody (ab65888) at 1/5000 dilutionLane 1 : Molecular weight markersLane 2 : SK-HEP-1 lysate at 30 µlLane 3 : SK-HEP-1 lysate at 20 µlLane 4 : SK-HEP-1 lysate at 10 µl

Anti-MASP1/3 antibody - ab65890 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MASP1/3 antibody (ab65890) at 1/5000 dilutionLane 1 : Molecular weight markersLane 2 : SK-HEP-1 Lysate at 30 µlLane 3 : SK-HEP-1 Lysate at 20 µlLane 4 : SK-HEP-1 Lysate at 10 µl

Anti-MASP1/3 antibody - ab65887 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MASP1/3 antibody - ab65889 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MASP1 antibody - ab65891 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab65891 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65891, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MASP1 antibody - ab72659 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MASP1 antibody (ab72659) at 1/500 dilutionLane 1 : Extracts from A549 cells, treated with etoposide (25uM, 24hours)Lane 2 : Extracts from A549 cells, treated with etoposide (25uM, 24hours) with Cl-peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-MASP1 antibody [MM0197-4F19] - ab89316 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MM0197-4F19

Anti-MASP1 antibody - Carboxyterminal end - ab65893 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MASP1 antibody - Catalytic domain - ab65892 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MASP3 antibody - Carboxyterminal end - ab65901 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MASP3 antibody - Carboxyterminal end (ab65901) at 1/1000 dilutionLane 1 : Molecular weight markersLane 2 : 80ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 3 : 40ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 4 : 20ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 5 : 10ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 6 : 5ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Observed band size : 43 kDa (why is the actual band size different from the predicted?)

Anti-MASP3 antibody - Catalytic domain - ab65900 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MASP3 antibody - Catalytic domain (ab65900) at 1/1000 dilutionLane 1 : Molecular weight markersLane 2 : 80ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 3 : 40ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 4 : 20ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 5 : 10ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Lane 6 : 5ng Recombinant Human MASP3 Catalytic Domain (aa450-721)Observed band size : 45 kDa (why is the actual band size different from the predicted?)

Anti-MASP1 / MASP Antibody (aa481-507, AP) - LS-C207219 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MASP1 / MASP Antibody (aa481-507, APC) - LS-C207220 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MASP1 / MASP Antibody (aa481-507, Biotin) - LS-C207222 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MASP1 / MASP Antibody (aa481-507, FITC) - LS-C207223 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Search more