ICC/IF image of ab137254 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab137254 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Dylight® 488 goat anti- rabbit (ab96899) IgG (H+L) preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Anti-MAST205 antibody (ab137254) at 1/500 dilution + K562 cell lysate at 30 µg