MAST2

Gene Symbol MAST2
Entrez Gene 23139
Alt Symbol MAST205, MTSSK
Species Human
Gene Type protein-coding
Description microtubule associated serine/threonine kinase 2
Other Description microtubule associated testis specific serine/threonine protein kinase|microtubule-associated serine/threonine-protein kinase 2
Swissprots Q8NDG1 Q5VT07 O94899 Q6P0Q8 Q9BYE8 Q96B94 Q5VT08 Q7LGC4
Accessions EAX06948 EAX06949 Q6P0Q8 AB018350 BAA34527 AB047005 BAB40778 AK027123 AK055291 BAG51495 AK096519 BAG53313 AK309510 AL137594 CAB70833 AL540100 AL833919 CAD38775 BC006166 AAH06166 BC015816 AAH15816 BC065499 AAH65499 BM675011 CN297081 XM_005270652 XP_005270709 XM_005270654 XP_005270711 XM_005270655 XP_005270712 XM_005270656 XP_005270713 XM_006710475 XP_006710538 XM_006710477 XP_006710540 XM_011541059 XP_011539361 XM_011541060 XP_011539362 XM_011541061 XP_011539363 XM_011541062 XP_011539364 XM_011541063 XP_011539365 XM_011541064 XP_011539366 XM_011541065 XP_011539367 XM_011541066 XP_011539368 XM_011541067 XP_011539369 XM_011541068 XP_011539370 XM_011541069 XP_011539371 NM_015112 NP_055927
Function Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm, cytoskeleton {ECO:0000269|PubMed:12117771}. Cell membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Recruited to the sub-membranous area on interaction with CDHR2.
Tissue Specificity Abundant in the testis. {ECO:0000269|PubMed:8902215}.

MAST205 (C-1) - sc-514198 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAST205 (C-1): sc-514198. Western blot analysis of MAST205 expression in K-562 (A), HeLa (B) and Jurkat (C) whole cell lysates.

MAST205 (H-300) - sc-134933 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MAST205 (H-300): sc-134933. Western blot analysis of MAST205 expression in K-562 (A) and HeLa (B) whole cell lysates.

MAST205 (P-20) - sc-55857 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MAST205 (T-20) - sc-55859 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-MAST205 antibody - ab137254 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab137254 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab137254 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Dylight® 488 goat anti- rabbit (ab96899) IgG (H+L) preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-MAST205 antibody - ab86412 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MAST205 antibody - ab57041 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57041 MAST205 antibody at 1ug/ml.

Anti-MAST205 / MAST2 Antibody (aa25-75) - LS-C288940 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MAST205 / MAST2 Antibody (aa1748-1798) - LS-C288941 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MAST205 / MAST2 Antibody (aa1154-1203) - LS-C292246 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MAST205 / MAST2 Antibody - LS-C309199 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-MAST205 / MAST2 Antibody (aa688-792) - LS-C309202 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-MAST205 / MAST2 Antibody (aa1201-1250) - LS-C119106 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from Jurkat/293/HepG2 cells, using MAST2 Antibody. The lane on the right is treated with the synthesized peptide.

MAST205 Antibody - NBP1-87900 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: MAST205 Antibody [NBP1-87900] Staining of human cell line A-431 shows positivity in cytoplasm.

MAST205 Antibody - NBP1-22986 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: MAST205 Antibody [NBP1-22986] - Whole cell lysate from HeLa cells. MAST2 was also immunoprecipitated by rabbit anti-MAST2 antibody NBP1-22985.

MAST205 Antibody - NBP1-22985 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: MAST205 Antibody [NBP1-22985] - Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-MAST2 antibody used for IP at 3 mcg/mg lysate.

MAST205 Antibody - H00023139-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: MAST205 Antibody [H00023139-B01P] - Analysis of MAST2 expression in transfected 293T cell line by MAST2 polyclonal antibody. Lane 1: MAST2 transfected lysate(87.12 KDa). Lane 2: Non-transfected lysate.

MAST205 Antibody (3F9) - H00023139-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F9
Sandwich ELISA: MAST205 Antibody (3F9) [H00023139-M01] - Detection limit for recombinant GST tagged MAST2 is 0.3 ng/ml as a capture antibody.

Anti-MAST2 antibody produced in rabbit - HPA040155 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MAST2: <B>Cat. No. HPA040155</B>: Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.

Anti-MAST2 antibody produced in mouse - SAB1401581 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Polyclonal Anti-MAST2, antibody produced in mouse, purified immunoglobulin: <B>Cat. No. SAB1401581</B>: Antibody concentration: 0.1 - 5.0 μg/mL. Specific band of ~87.12 kDa using MAST2 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Monoclonal Anti-MAST2 antibody produced in mouse - WH0023139M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F9
<B>Immunoblotting</B><BR/>Monoclonal Anti-MAST2, <B>Cat. No. WH0023139M1</B> used at the antibody concentration: 1 μg/mL. Specific band of ~34.55 kDa using immunogen protein lysate.

Anti-MAST2 antibody produced in rabbit - HPA039722 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MAST2: <B>Cat. No. HPA039722</B>: Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.

Anti-MAST2 antibody produced in rabbit - SAB4500947 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MAST2: <B>Cat. No. SAB4500947</B>: Immunoblotting analysis of extracts from Jurkat/293/HepG2 cells, using Anti-MAST2 antibody. The lane on the left was treated with the Anti-MAST2 antibody. The lane on the right (negative control) was treated with both Anti-MAST2 antibody and the synthesized immunogen peptide.

MAST2 Antibody (OAAF02502) - OAAF02502 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

MAST2 Antibody (OAAF06608) - OAAF06608 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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