Anti-MAVS antibody

• Supplier: Abcam
• Catalog: ab25084
• Prices: $390.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IP WB ICC/IF ICC/IF IHC-P
• Species Reactivities: Human, Bovine
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human MAVS
• Description: Rabbit Polyclonal
• Gene: MAVS
• Conjugate: Unconjugated

Product images

MAVS was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to MAVS and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab25084.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 70kDa: MAVS

All lanes : Anti-MAVS antibody (ab25084) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

ICC/IF image of ab25084 stained human HEK293 cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab25084, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence.  5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

IHC image of MAVS staining in human liver carcinoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25084, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product References

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NLRX1 is a regulator of mitochondrial antiviral immunity. - NLRX1 is a regulator of mitochondrial antiviral immunity.

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Hepatitis A virus protein 2B suppresses beta interferon (IFN) gene transcription - Hepatitis A virus protein 2B suppresses beta interferon (IFN) gene transcription

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The mitochondrial antiviral signaling protein, MAVS, is cleaved during apoptosis. - The mitochondrial antiviral signaling protein, MAVS, is cleaved during apoptosis.

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