Anti-MAVS antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab31334
• Prices: $401.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ChIP ICC/IF ICC/IF WB IP
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide derived from within residues 100 - 200 of Human MAVS
• Description: Rabbit Polyclonal
• Gene: MAVS
• Conjugate: Unconjugated

Product images

All lanes : Anti-MAVS antibody - ChIP Grade (ab31334) at 1 µg/mlLane 1 : Hela Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lysates/proteins at 20 µg per lane.SecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilution

ICC/IF image of ab31334 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31334, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

ab31334 staining MAVS in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% NGS, 5% BSA in PBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in diluent) for 16 hours at 4°C. A Goat anti-rabbit HRP polyclonal (1/250) was used as the secondary antibody.See Abreview

MAVS was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal MAVS and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31334.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 75kDa: MAVS

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