Anti-MBD3 antibody [EPR9912] (ab155105) at 1/5000 dilution (purified) + F9 cell lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
All lanes : Anti-MBD3 antibody [EPR9912] (ab155105) at 1/5000 dilution (purified)Lane 1 : HEK293 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
All lanes : Anti-MBD3 antibody [EPR9912] (ab155105) at 1/1000 dilution (unpurified)Lane 1 : F9 cell lysateLane 2 : C6 cell lysateLane 3 : HeLa cell lysateLane 4 : 293T cell lysateLane 5 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution