MBD3

Gene Symbol MBD3
Entrez Gene 53615
Alt Symbol -
Species Human
Gene Type protein-coding
Description methyl-CpG binding domain protein 3
Other Description methyl-CpG-binding domain protein 3
Swissprots Q86XF4 Q6PJZ9 D6W5Z2 O95983 A8K4B7 Q6PIL9
Accessions AAC72104 EAW69477 EAW69478 EAW69479 EAW69480 EAW69481 O95983 AA576859 AF072247 AAC68876 AK001474 BAG50920 AK021532 AK092508 AK290882 BAF83571 AL390153 BC000872 BC009372 AAH09372 BC009438 AAH09438 BC018784 AAH18784 BC032443 AAH32443 BC043619 AAH43619 BM684485 BU181763 X58233 CAA41191 NM_001281453 NP_001268382 NM_001281454 NP_001268383 NM_003926
Function Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases. {ECO:0000269|PubMed:10947852, ECO:0000269|PubMed:12124384, ECO:0000269|PubMed:18644863, ECO:0000269|PubMed:23361464, ECO:0000269|PubMed:24307175, ECO:0000269|PubMed:9774669}.
Subcellular Location Nucleus. Chromosome. Note=Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes.

MBD3 Antibody - 3896 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NTERA-22, F9 and C6 cells using MBD3 Antibody.

MBD2/3 (H-50) - sc-28743 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MBD2/3 (H-50): sc-28743. Western blot analysis of MBD2 and MBD3 expression in Jurkat (A), HeLa (B), A-431 (C), RAW 264.7 (D), NIH/3T3 (E) and U-937 (F) nuclear extracts.

MBD3 (C-18) - sc-9402 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MBD3 (C-18): sc-9402. Western blot analysis of MBD3 expression in HeLa (A) and Jurkat (B) nuclear extracts.

MBD3 (F-11) - sc-166319 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MBD3 (F-11): sc-166319. Western blot analysis of MBD3 expression in HeLa (A) and Jurkat (B) whole cell lysates.

MBD3 (N-20) - sc-9400 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-MBD3 antibody - ab16057 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MBD3 antibody (ab16057) at 0.11 µg/mlLane 1 : HeLaLane 2 : Hela nuclear Lane 3 : A431Lane 4 : HeLa with Human MBD3 peptide (ab16210) at 1 µg/mlLane 5 : HeLa nuclear with Human MBD3 peptide (ab16210) at 1 µgLane 6 : A431 with Human MBD3 peptide (ab16210) at 1 µg/mlSecondaryAlexa Fluor Goat polyclonal anti-Rabbit IgG (700)

Anti-MBD3 antibody - ab91458 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MBD3 with ab91458 at 1/1000 (1µg/ml). Detection: DAB.

Anti-MBD3 antibody - ab69887 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MBD3 antibody (ab69887) at 1/500 dilutionLane 1 : Extracts from Jurkat cellsLane 2 : Extracts from Jurkat cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-MBD3 antibody - ab89440 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-MBD3 antibody (ab89440) at 1 µg/ml + 293 cell lysate at 50 µgdeveloped using the ECL technique

Anti-MBD3 antibody - ab124467 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MBD3 antibody (ab124467) at 1/1000 dilution + 293T cell lysate

Anti-MBD3 antibody [EPR9912] - ab155105 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9912
Anti-MBD3 antibody [EPR9912] (ab155105) at 1/5000 dilution (purified) + F9 cell lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-MBD3 antibody [EPR9913] (Alexa Fluor® 488) - ab197858 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9913
ab197858 staining MBD3 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197858 at 1/500 dilution (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed, at 1µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-MBD3 antibody - Carboxyterminal end - ab71777 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MBD3 antibody - Carboxyterminal end (ab71777) at 1/100 dilutionLane 1 : Non-transfected 293 cell lysatesLane 2 : MBD3 transfected 293 cell lysatesLysates/proteins at 2 µg per lane.

Anti-MBD3 Antibody - LS-C37316 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-MBD3 Antibody - LS-C72249 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-MBD3 Antibody (aa1-203) - LS-C178924 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MBD3 Antibody (aa220-234, clone 3A3) - LS-C193584 from LifeSpan Bioscience

Prices $905.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3A3

Anti-MBD3 Antibody (aa72-121) - LS-C101826 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MBD3 antibody LS-C101826 Western blot of Transfected 293T cell lysate.

Anti-MBD3 Antibody (His250) - LS-C176892 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of MBD3 (H250) pAb in extracts from Jurkat cells.

Anti-MBD3 Antibody (aa1-30, APC) - LS-C215749 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MBD3 Antibody (aa221-270) - LS-C118742 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from Jurkat cells, using MBD3 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-MBD3 Antibody (aa1-30, AP) - LS-C215748 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MBD3 Antibody (aa1-30, Biotin) - LS-C215750 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MBD3 Antibody (aa1-30, Azide-free, HRP) - LS-C215751 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MBD3 Antibody (aa1-30, FITC) - LS-C215752 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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