
Flow cytometric analysis of HeLa cells labeling MCAK with ab187652 at 1/260 dilution compared to negative control cells. Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.
![All lanes : Anti-MCAK antibody [EPR14838] (ab187652) at 1/1000 dilutionLane 1 : MCF-7 cell lysateLane 2 : 293T cell lysateLane 3 : Hela cell lysateLane 4 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/20170_ab187652-221182-wb.jpg)
All lanes : Anti-MCAK antibody [EPR14838] (ab187652) at 1/1000 dilutionLane 1 : MCF-7 cell lysateLane 2 : 293T cell lysateLane 3 : Hela cell lysateLane 4 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Immunofluorscence analysis of MCF7 cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.

Immunofluorscence analysis of HeLa cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.