Anti-MCL1 antibody

• Supplier: Abcam
• Catalog: ab53709
• Prices: $385.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P ELISA ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide derived from human MCL1
• Description: Rabbit Polyclonal
• Gene: MCL1
• Conjugate: Unconjugated

Product images

All lanes : Anti-MCL1 antibody (ab53709) at 1/500 dilutionLane 1 : Extracts from HuvEc cells minus the immunising peptideLane 2 : Extracts from HuvEc cells plus the immunising peptide

ab53709, at a 1/50 dilution, minus immunising peptide, staining Human MCL1 in Breast Carcinoma, using Immunohistochemistry, Paraffin embedded tissue.

ab53709, at a 1/50 dilution, plus immunising peptide, staining Human MCL1 in Breast Carcinoma, using Immunohistochemistry, Paraffin embedded tissue.

ICC/IF image of ab53709 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53709, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Preapoptotic cell stress response of primary hepatocytes. - Preapoptotic cell stress response of primary hepatocytes.

Nipic D, Pirc A, Banic B, Suput D, Milisav I. Hepatology. 2010 Jun;51(6):2140-51.

Regulation of serum response factor activity and smooth muscle cell apoptosis by - Regulation of serum response factor activity and smooth muscle cell apoptosis by

Rodenberg JM, Hoggatt AM, Chen M, Touw K, Jones R, Herring BP. Am J Physiol Cell Physiol. 2010 Nov;299(5):C1058-67. doi: