Cleaved Caspase-3 (Asp175) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 9661
• Prices: $109.00, $287.00, $678.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IP IHC-P IHC-F ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Monkey, Bovine, Dog, Pig
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to (Asp175) in human caspase-3.
• Description: Rabbit Polyclonal
• Gene: CASP3

Product images

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells untreated, staurosporine-treated (3hrs, 1 µM in vivo) or cytochrome c-treated (1hr, 0.25 mg/ml in vitro), using Caspase-3 Antibody #9662 (upper) or Cleaved Caspase-3 (Asp175) Antibody (lower).

Immunohistochemical analysis of paraffin-embedded human tonsil, showing cytoplasmic and perinuclear localization in apoptotic cells (low and high magnification), using Cleaved Caspase-3 (Asp175) Antibody.

Immunohistochemical analysis using Cleaved caspase-3 (Asp175) antibody on SignalSlide™ Cleaved Caspase-3 IHC controls #8104 (paraffin-embedded Jurkat cells, untreated (left) or etoposide-treated (right)).

Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) Antibody preincubated with control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide #1050 (right).

Immunohistochemical analysis of frozen H1650 xenograft section, using Cleaved Caspase-3 (Asp175) Antibody.

Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine #9953 treated (right), labeled with Cleaved Caspase-3 (Asp175) Antibody (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin #8953 (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with etoposide #2200 (green), using Cleaved Caspase-3 (Asp175) Antibody compared to a nonspecific negative control antibody (red).