CASP3

Gene Symbol CASP3
Entrez Gene 836
Alt Symbol CPP32, CPP32B, SCA-1
Species Human
Gene Type protein-coding
Description caspase 3, apoptosis-related cysteine peptidase
Other Description CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine protease|caspase-3|cysteine protease CPP32|procaspase3|protein Yama
Swissprots Q96AN1 Q96KP2 D3DP53 P42574 A8K5M2
Accessions AAO25654 CAG17895 CAV28500 CBX53863 CBX53864 EAX04673 EAX04674 EAX04675 EAX04676 P42574 AJ413269 CAC88866 AK291337 BAF84026 AU127028 AU133051 BC016926 AAH16926 BU753483 BX647609 CAI46084 DQ891543 ABM82469 DQ894731 ABM85657 U13737 AAA65015 U13738 AAB60355 U26943 AAA74929 XM_011532301 XP_011530603 NM_004346 NP_004337 NM_032991 NP_116786
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. {ECO:0000269|PubMed:21357690, ECO:0000269|PubMed:7596430}.
Subcellular Location Cytoplasm.
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Top Pathways TNF signaling pathway, Apoptosis, Viral carcinogenesis, Non-alcoholic fatty liver disease (NAFLD), MicroRNAs in cancer

Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) - 9654 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 5A1E
Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hrs), using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) and detected with Streptavidin-HRP #3999.

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (PE Conjugate) - 12768 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D3E9
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with Etoposide #2200 (green), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (PE Conjugate).

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 9603 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D3E9
Confocal immunofluorescent analysis of HeLa cells, untreated (left), or treated with staurosporine #9953 (right), using Cleaved Caspase-3 (Asp175) (D3E9) (Alexa Fluor® 488 Conjugate) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Pacific Blue™ Conjugate) - 8788 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D3E9
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Pacific Blue™ Conjugate).

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor ® 555 Conjugate) - 9604 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D3E9
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (1 μM, 4 hr; right), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor ® 555 Conjugate) (red). Actin filaments were labeled with Alexa Fluor ® 488 phalloidin (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 9602 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D3E9
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (1 μM, 4 hr; right), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor ® 488 Phalloidin #8878 (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor ® 488 Conjugate) - 9669 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Polyclonal
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or staurosporine-treated (right), using Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin #8953 (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (PE Conjugate) - 9978 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 5A1E
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (PE Conjugate).

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor ® 594 Conjugate) - 8172 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D3E9
Immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (1 μM, 4 hr; right), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor ® 594 Conjugate) (red). Actin filaments were labeled with Alexa Fluor ® 488 phalloidin #8878 (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Apoptosis Marker: Cleaved Caspase-3 (Asp175) Antibody (Fluorescein Conjugate) - 9667 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Cleaved Caspase-3 (Asp175) Antibody - 9661 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells untreated, staurosporine-treated (3hrs, 1 µM in vivo) or cytochrome c-treated (1hr, 0.25 mg/ml in vitro), using Caspase-3 Antibody #9662 (upper) or Cleaved Caspase-3 (Asp175) Antibody (lower).

SignalStain ® Apoptosis (Cleaved Caspase-3) IHC Detection Kit - 12692 from Cell Signaling Technology

Prices $472.00
Sizes 1 kit (120 slides)
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded E14 mouse embryo using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579 (left) or Rabbit (DA1E) mAb IgG XP ® SignalStain ® Isotype Control #12960 (right).

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb - 9579 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (40 assays), 100 µl (200 assays)
Host Rabbit
Clonality Monoclonal
Clone D3E9
Immunohistochemical analysis of paraffin-embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb on SignalSlide ® Cleaved Caspase-3 (Asp175) IHC Controls #8104.

Apoptosis Marker: Cleaved Caspase-3 (Asp175) Western Detection Kit - 9660 from Cell Signaling Technology

Prices $489.00
Sizes 1 kit (10 western blots)
Clonality Polyclonal

Caspase-3 (8G10) Rabbit mAb - 9665 from Cell Signaling Technology

Prices $99.00, $261.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 8G10
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® Caspase-3 siRNA II (+), using Caspase-3 (8G10) Rabbit mAb and α-Tubulin (11H10) Rabbit mAb #2125. Caspase-3 (8G10) Rabbit mAb confirms silencing of caspase-3 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of caspase-3 siRNA.

Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb - 9664 from Cell Signaling Technology

Prices $109.00, $307.00, $719.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 5A1E
Western blot analysis of extracts from C6 (rat), NIH/3T3 (mouse), and Jurkat (human) cells, untreated or treated with staurosporine #9953 (1uM, 3hrs) or etoposide #2200 (25uM, 5hrs) as indicated, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

Caspase-3 Antibody - 9662 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25uM, 5hrs), and NIH/3T3 cells, untreated or staurosporine-treated (1uM, 3hrs), using Caspase-3 Antibody.

Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated) - 14214 from Cell Signaling Technology

Prices $250.00
Sizes 100 µl (300 sections)
Host Rabbit
Clonality Monoclonal
Clone D3R6Y
Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma in situ using Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated).

Caspase-3 (3G2) Mouse mAb - 9668 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 3G2
Western blot analysis of HeLa cell extracts treated with 1uM staurosporine (3hrs), using Caspase-3 (3G2) Mouse mAb.

Caspase-3 (D3R6Y) Rabbit mAb - 14220 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3R6Y
Western blot analysis of various cell lines, untreated (-) or treated with Staurosporine #9953 (1 μM; 3 hr) or with Etoposide #2200 (25 μM, overnight), using Caspase-3 (D3R6Y) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). MCF7 cells are negative for caspase-3 expression.

caspase-3 (3CSP01) - sc-65496 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
caspase-3 (3CSP01): sc-65496. Western blot analysis of caspase-3 expression in non-transfected: sc-117752 (A) and human caspase-3 transfected: sc-113427 (B) 293T whole cell lysates.

caspase-3 (4.1.18) - sc-65497 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
caspase-3 (4.1.18): sc-65497. Western blot analysis of caspase-3 expression in CCRF-CEM (A), CCRF-HSB-2 (B) and Jurkat (C) whole cell lysates.

caspase-3 (7.1.44) - sc-56051 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
caspase-3 (7.1.44): sc-56051. Western blot analysis of caspase-3 expression in non-transfected: sc-117752 (A) and human caspase-3 transfected: sc-113427 (B) 293T whole cell lysates.

caspase-3 (E-8) - sc-7272 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
caspase-3 (E-8): sc-7272. Western blot analysis of caspase-3 expression in CCRF-CEM whole cell lysate.
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